Lutz E M, Sheward W J, West K M, Morrow J A, Fink G, Harmar A J
Department of Pharmacology, University of Edinburgh, UK.
FEBS Lett. 1993 Nov 8;334(1):3-8. doi: 10.1016/0014-5793(93)81668-p.
We have cloned and sequenced a cDNA (RPR4) encoding a new member of the secretin/calcitonin/parathyroid hormone (PTH) receptor family. RPR4 was identified by PCR of rat pituitary cDNA, and a full-length clone was isolated from a rat olfactory bulb cDNA library. When RPR4 was functionally expressed in COS 7 cells, cyclic adenosine monophosphate (cAMP) production was stimulated by vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptides (PACAP-38 and PACAP-27) and helodermin, with equal potency. Peptide histidine isoleucine (PHI) and rat growth hormone releasing hormone (rGHRH) also stimulated cAMP production at lower potency. This suggests that RPR4 encodes a novel VIP receptor which we have designated the VIP2 receptor. In situ hybridisation showed that mRNA for this receptor was present mainly in the thalamus, hippocampus and in the suprachiasmatic nucleus.
我们克隆并测序了一个编码促胰液素/降钙素/甲状旁腺激素（PTH）受体家族新成员的cDNA（RPR4）。通过大鼠垂体cDNA的PCR鉴定出RPR4，并从大鼠嗅球cDNA文库中分离出一个全长克隆。当RPR4在COS 7细胞中进行功能性表达时，血管活性肠肽（VIP）、垂体腺苷酸环化酶激活肽（PACAP - 38和PACAP - 27）和海蜥蜴毒素能以相同效力刺激环磷酸腺苷（cAMP）的产生。肽组氨酸异亮氨酸（PHI）和大鼠生长激素释放激素（rGHRH）也能以较低效力刺激cAMP的产生。这表明RPR4编码一种新型VIP受体，我们将其命名为VIP2受体。原位杂交显示该受体的mRNA主要存在于丘脑、海马体和视交叉上核中。
