来自苏云金芽孢杆菌的两种免疫抑制剂干扰天蚕蛾蛹体液防御系统的证据。
Evidence for two immune inhibitors from Bacillus thuringiensis interfering with the humoral defense system of saturniid pupae.
作者信息
Edlund T, Sidén I, Boman H G
出版信息
Infect Immun. 1976 Oct;14(4):934-41. doi: 10.1128/iai.14.4.934-941.1976.
Abstract
Mutants of Bacillus thuringiensis lacking either beta-exotoxin or gamma-endotoxin were compared for their virulence using pupae of a giant silk moth. Known doses of viable log-phase bacteria were injected, and the response was followed as the number of viable bacteria in the hemolymph. The results obtained imply that, in the system used, neither the beta-exotoxin nor the gamma-endotoxin and the sporeforming ability are of importance for virulence. Results with sterile culture filtrate from B. thuringiensis have given evidence for the production of two inhibitors, A and B, which interfere with the humoral defense system in pupae of Hyalophora cecropia. Inhibitor A, which blocked the lysis of Escherichia coli,was precipitated by trichloroacetic acid and sensitive to heating. Inhibitor B, which blocked the killing of Bacillus cereus, was soluble in trichloroacetic acid and resistant to 90 degrees C for 5 min. Both inhibitors are believed to contribute to the insecticidal nature of B. thuringiensis.
摘要
利用大蚕蛾的蛹，对缺乏β-外毒素或γ-内毒素的苏云金芽孢杆菌突变体的毒力进行了比较。注射已知剂量的对数期活菌，以血淋巴中活菌数量作为指标跟踪反应情况。所得结果表明，在所使用的系统中，β-外毒素、γ-内毒素以及芽孢形成能力对毒力均不重要。苏云金芽孢杆菌无菌培养滤液的实验结果证明，其可产生两种抑制剂A和B，它们会干扰天蚕蛹的体液防御系统。抑制大肠杆菌裂解的抑制剂A可被三氯乙酸沉淀，且对加热敏感。抑制蜡样芽孢杆菌杀灭的抑制剂B可溶于三氯乙酸，且在90℃下5分钟仍具有抗性。两种抑制剂都被认为与苏云金芽孢杆菌的杀虫特性有关。
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