Heierson A, Sidén I, Kivaisi A, Boman H G
J Bacteriol. 1986 Jul;167(1):18-24. doi: 10.1128/jb.167.1.18-24.1986.
Starting from a crystal-negative parental strain of Bacillus thuringiensis, we isolated certain bacteriophage-resistant mutants which showed decreased virulence in pupae of the cecropia moth (Hyalophora cecropia). These strains (class I mutants) were highly pleiotropic and showed resistance to seven or eight different phages, sensitivity to methicillin, and loss of flagella. They were also more sensitive to cecropia immune hemolymph in vitro. In addition, the export of at least three proteins was reduced. Revertants (class II mutants) were sensitive to phages, virulent, and resistant to penicillin derivatives. One class II mutant was a complete revertant in all properties examined. The other class II mutant was an incomplete revertant still susceptible to immune hemolymph and with repressed export of proteins. Virulence was not coupled to phage resistance as such or to lack of flagella because other mutants affected in these properties were virulent. Other factors which could be excluded as causes of virulence were production of extracellular protease and hemolysin.
从苏云金芽孢杆菌的一个晶体阴性亲本菌株出发，我们分离出了某些抗噬菌体突变体，这些突变体在天蚕蛾（大透目天蚕蛾）蛹中的毒力降低。这些菌株（I类突变体）具有高度多效性，对七种或八种不同的噬菌体具有抗性，对甲氧西林敏感，且失去鞭毛。它们在体外对天蚕免疫血淋巴也更敏感。此外，至少三种蛋白质的输出减少。回复突变体（II类突变体）对噬菌体敏感、具有毒力且对青霉素衍生物具有抗性。一个II类突变体在所有检测的特性上都是完全回复突变体。另一个II类突变体是不完全回复突变体，仍然对免疫血淋巴敏感且蛋白质输出受到抑制。毒力与噬菌体抗性本身或鞭毛缺失并无关联，因为在这些特性上受到影响的其他突变体具有毒力。可以排除作为毒力原因的其他因素是细胞外蛋白酶和溶血素的产生。
