Tohgi H, Abe T, Yamazaki K, Murata T, Isobe C, Ishizaki E
Department of Neurology, Iwate Medical University, Morioka, Japan.
J Neural Transm (Vienna). 1998;105(10-12):1283-91. doi: 10.1007/s007020050131.
The concentration of the nitric oxide (NO) metabolites nitrite (NO2-) and nitrate (NO3-) in the cerebrospinal fluid from patients with Alzheimer's disease (AD), vascular dementia of the Binswanger type (VDBT) or multiple small infarct type (MSID), and controls were determined using high performance liquid chromatography. The nitrite concentration was significantly higher in VDBT/MSID patients than in controls (p < 0.005). The nitrate concentration and the combined nitrite and nitrate concentration was significantly higher in both AD (p < 0.05) and VDBT/MSID (p < 0.001) patients than in controls, with these concentrations being significantly greater in VDBT/MSID than AD patients (p < 0.005). The combined nitrite and nitrate concentration significantly decreased as the severity of dementia progressed in AD (rs=0.70, p < 0.01), but remained elevated in all stages of VDBT/MSID. These results suggest that NO production or oxidation in the brain increases in the early stage of AD and then decreases as neuronal cell loss progresses, but increases throughout the course of disease in VDBT/MSID, which may in part contribute to neuronal degeneration in both conditions.
采用高效液相色谱法测定了阿尔茨海默病（AD）患者、宾斯旺格型血管性痴呆（VDBT）或多发性小梗死型（MSID）患者以及对照组脑脊液中一氧化氮（NO）代谢产物亚硝酸盐（NO2-）和硝酸盐（NO3-）的浓度。VDBT/MSID患者的亚硝酸盐浓度显著高于对照组（p < 0.005）。AD患者（p < 0.05）和VDBT/MSID患者（p < 0.001）的硝酸盐浓度以及亚硝酸盐与硝酸盐的联合浓度均显著高于对照组，且VDBT/MSID患者的这些浓度显著高于AD患者（p < 0.005）。在AD患者中，随着痴呆严重程度的进展，亚硝酸盐与硝酸盐的联合浓度显著降低（rs = 0.70，p < 0.01），但在VDBT/MSID的各个阶段均保持升高。这些结果表明，AD早期大脑中NO的产生或氧化增加，随后随着神经元细胞丢失的进展而减少，但在VDBT/MSID病程中持续增加，这可能在一定程度上导致了两种情况下的神经元变性。
