恒河猴的淋巴细胞分离、花环形成及丝裂原刺激
Lymphocyte isolation, rosette formation, and mitogen stimulation in Rhesus monkeys.
作者信息
Taylor D W, Marchette N J, Siddiqui W A
出版信息
Dev Comp Immunol. 1978 Jul;2(3):539-46. doi: 10.1016/s0145-305x(78)80014-7.
PMID:99337
Abstract
Over 70% of rhesus monkey peripheral mononuclear cells were isolated on sodium metrizoate-ficoll gradients with greater than 98% purity. Rhesus blood contained 47.8% active E, 58.2% total E, and 30.2% EAC rosette forming cells. Optimal conditions for mitogen studies were determined using phytohemagglutinin, concanavalin A, pokeweed mitogen, lipopolysaccharide and streptolysin O.
摘要
超过70%的恒河猴外周血单个核细胞通过泛影葡胺-聚蔗糖梯度分离，纯度大于98%。恒河猴血液中含有47.8%的活性E、58.2%的总E以及30.2%的EAC花环形成细胞。使用植物血凝素、刀豆蛋白A、商陆有丝分裂原、脂多糖和链球菌溶血素O确定了有丝分裂原研究的最佳条件。
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