Wong K K
Planta Med. 1998 Dec;64(8):756-7. doi: 10.1055/s-2006-957575.
The mechanism of the aortic relaxation induced by a low concentration of berberine was investigated in rat isolated aorta. It was found that the aortic relaxation induced by berberine below 1 x 10(-6) M was solely endothelium dependent. Denuded aorta and methylene blue pretreated aorta did not demonstrate aortic relaxation with such berberine concentrations. Higher concentrations (above 1 x 10(-6) M berberine), however, induced aortic relaxation irrespective of the presence of intact endothelium or methylene blue pretreatment. Since methylene blue is known as a direct inhibitor of nitric oxide synthase beside being a nonspecific guanylyl cyclase inhibitor, the present data suggest that the mechanism of the aortic relaxation induced by a low berberine concentration is solely dependent on the presence of endothelium.
在大鼠离体主动脉中研究了低浓度黄连素诱导主动脉舒张的机制。结果发现，低于1×10(-6)M的黄连素诱导的主动脉舒张完全依赖于内皮。去内皮的主动脉和亚甲蓝预处理的主动脉在该黄连素浓度下未表现出主动脉舒张。然而，较高浓度（高于1×10(-6)M黄连素）诱导的主动脉舒张与完整内皮的存在或亚甲蓝预处理无关。由于亚甲蓝除了是一种非特异性鸟苷酸环化酶抑制剂外，还是一氧化氮合酶的直接抑制剂，目前的数据表明低浓度黄连素诱导主动脉舒张的机制仅依赖于内皮的存在。
