用十二烷基硫酸钠处理后溶解的4种乳脂肪球膜蛋白的分离、制备及氨基酸组成

Isolation, preparation and the amino acid composition of 4 milk-fat globule membrane proteins solubilized by treatment with sodium dodecyl sulphate.

作者信息

Cawston T E, Anderson M, Cheeseman G C

出版信息

J Dairy Res. 1976 Oct;43(3):401-9. doi: 10.1017/s0022029900015983.

DOI:10.1017/s0022029900015983

Abstract

Milk-fat globule membrane (MFGM) proteins were solubilized by treatment with SDS. Four of the major proteins were isolated as SDS complexes using column chromatography. The purity of each isolate was determined by SDS polyacrylamide gel electrophoresis and sufficient of each protein was obtained for amino acid analysis. The amino acid compositions of the isolated MFGM proteins and a total MFGM protein extract were determined. Differences in amino acid composition were found in particular between the major MFGM glycoprotein and the other 3 membrane proteins. The relationships of the amino acid composition to protein properties and structure are discussed.

摘要

乳脂肪球膜（MFGM）蛋白经SDS处理后可溶解。使用柱色谱法将四种主要蛋白质分离为SDS复合物。通过SDS聚丙烯酰胺凝胶电泳测定每种分离物的纯度，并获得足够的每种蛋白质用于氨基酸分析。测定了分离的MFGM蛋白和总MFGM蛋白提取物的氨基酸组成。特别是在主要的MFGM糖蛋白与其他三种膜蛋白之间发现了氨基酸组成的差异。讨论了氨基酸组成与蛋白质性质和结构的关系。