Imir T, Saksela E, Mäkelä O
J Immunol. 1976 Nov;117(5 Pt.2):1938-42.
Haptenated chicken erythrocytes, anti-hapten antibodies and normal spleen cells were used for a sutdy of parameters of antibody-dependent cell-mediated cytotoxicity (ADCC). Anti-hapten antibody was introduced to the reaction either bound to the target cells (sensitization) or to the effector cells (arming). Our data suggest that the same effector cells and the same antibodies were responsible for both types of reactions. More antibody was required for arming (ca. 1 ng/ml) than for sensitization (ca. 10 pg/ml). Antibodies of several mammalian species could cooperate in ADCC with human, rat, or guinea pig spleen effector cells, but fowl antibodies were inefficient in the arming of mammalian effector cells. Rat and human spleen cells were powerful effector cells whereas guinea pig, rabbit, and mouse spleens yielded weakly cytotoxic suspensions. Rabbit antibodies, on the other hand, were efficient, but rat antibodies were less efficient in ADCC. Mouse and guinea pig antibodies appear to take an intermediate position. Our data suggest that ADCC effector cells of the spleen contain phagocytic and nonphagocytic cells, and the non-phagocytic effector cells still constitute a heterogenous population.
用半抗原化鸡红细胞、抗半抗原抗体和正常脾细胞进行抗体依赖性细胞介导的细胞毒性（ADCC）参数研究。抗半抗原抗体以与靶细胞结合（致敏）或与效应细胞结合（武装）的方式引入反应体系。我们的数据表明，两种反应类型均由相同的效应细胞和相同的抗体介导。武装反应（约1 ng/ml）所需的抗体量比致敏反应（约10 pg/ml）更多。几种哺乳动物的抗体可与人类、大鼠或豚鼠脾效应细胞协同参与ADCC，但禽类抗体对哺乳动物效应细胞的武装作用效率较低。大鼠和人类脾细胞是强大的效应细胞，而豚鼠、兔和小鼠的脾脏产生的细胞毒性悬浮液较弱。另一方面，兔抗体在ADCC中效率较高，但大鼠抗体效率较低。小鼠和豚鼠抗体似乎处于中间位置。我们的数据表明，脾脏的ADCC效应细胞包含吞噬细胞和非吞噬细胞，且非吞噬性效应细胞仍然构成一个异质性群体。
