Kagnoff M F, Campbell S
Gastroenterology. 1976 Mar;70(3):341-6.
Lymphoid cells from nonsensitized donors can be cytotoxic in vitro for target cells coated with antibody. The antibody can be directed either against antigens which are intrinsic to or experimentally attached to the target cell surface. Killing of target cells by this method has been termed antibody-dependent cell-mediated cytotoxicity (ADCC). In these investigations we compared the ability of Peyer's patch cells and spleen cells from nonsensitized mice to lyse lipoplysaccharide-coated and uncoated target cells by an ADCC mechanism. The results demonstrate that spleen cells, but not Peyer's patch cells, from identical mice can be cytotoxic for lipopolysaccharide-coated chicken erythrocytes (CRBC) in the presence of anti-lipopolysaccharide antibody and uncoated CRBC in the presence of anti-CRBC antibody. Thus, Peyer's patches in mice appear to be deficient in the effector cell type(s) required to kill antibody-coated erythrocyte target cells by an ADCC mechanism as well as the macrophage-like accessory adherent cell type(s) required for the induction of humoral antibody synthesis and cell-mediated cytotoxic allograft reactions in vitro.
来自未致敏供体的淋巴细胞在体外可对包被有抗体的靶细胞产生细胞毒性。该抗体可以针对靶细胞表面固有的抗原，也可以针对通过实验附着于靶细胞表面的抗原。通过这种方法杀伤靶细胞被称为抗体依赖性细胞介导的细胞毒性（ADCC）。在这些研究中，我们比较了来自未致敏小鼠的派伊尔结细胞和脾细胞通过ADCC机制裂解脂多糖包被和未包被的靶细胞的能力。结果表明，来自相同小鼠的脾细胞而非派伊尔结细胞，在存在抗脂多糖抗体时可对脂多糖包被的鸡红细胞（CRBC）产生细胞毒性，在存在抗CRBC抗体时可对未包被的CRBC产生细胞毒性。因此，小鼠的派伊尔结似乎缺乏通过ADCC机制杀伤抗体包被的红细胞靶细胞所需的效应细胞类型，以及在体外诱导体液抗体合成和细胞介导的细胞毒性同种异体移植反应所需的巨噬细胞样辅助黏附细胞类型。
