Wåhlin B, Perlmann H, Perlmann P
J Exp Med. 1976 Nov 2;144(5):1375-80. doi: 10.1084/jem.144.5.1375.
When monolayers of bovine erythrocytes (Eb) were exposed to purified human blood lymphocytes and either IgG or IgM fractions of rabbit anti-Eb serum, clear zones (plaques) appeared when Eb had been lysed by antibody-dependent effector cells (K cells). IgG-dependent plaque formation was complete by 20 h of incubation, while the IgM-dependent reaction required 40 h. The estimated minimal numbers of plaque forming cells (PFC) were 5.6% (IgG) and 2.0% (IgM) of the added lymphocytes. Inhibition experiments with human IgG or IgM indicated that different immunoglobulin receptors on the effector cells were involved in the two systems. In the IgG system, approximately 50% of the PFC had complement receptors and approximately 30% receptors for Helix pomatia A hemagglutinin (HP). In the IgM system, less than 10% of the PFC had complement receptors, while approximately 60% had HP receptors. The results suggest that a subset of human T cells had IgM-dependent K-cell potential. These cells are different from the majority of the IgG-dependent K cells.
当牛红细胞（Eb）单层暴露于纯化的人血淋巴细胞以及兔抗-Eb血清的IgG或IgM组分时，当Eb被抗体依赖性效应细胞（K细胞）裂解时会出现清晰的区域（蚀斑）。IgG依赖性蚀斑形成在孵育20小时时完成，而IgM依赖性反应需要40小时。估计蚀斑形成细胞（PFC）的最小数量分别为所添加淋巴细胞的5.6%（IgG）和2.0%（IgM）。用人IgG或IgM进行的抑制实验表明，效应细胞上不同的免疫球蛋白受体参与了这两个系统。在IgG系统中，约50%的PFC具有补体受体，约30%具有光滑双脐螺血凝素（HP）受体。在IgM系统中，不到10%的PFC具有补体受体，而约60%具有HP受体。结果表明，人T细胞的一个亚群具有IgM依赖性K细胞潜能。这些细胞与大多数IgG依赖性K细胞不同。
