镁离子与磷壁酸的相互作用。
The interaction of magnesium ions with teichoic acid.
作者信息
Lambert P A, Hancock I C, Baddiley J
出版信息
Biochem J. 1975 Sep;149(3):519-24. doi: 10.1042/bj1490519.
Abstract
The binding of Mg2+ to the wall teichoic acid of Lactobacillus buchneri N.C.I.B. 8007 was measured by equilibrium dialysis at controlled ionic concentration and pH. In an aqueous solution containing 10mM-NaCl at pH 5.0 one Mg2+ ion was bound for every two phosphate groups of the teichoic acid, with an apparent association constant, Kassoc. = 2.7 x 10(3) M-1. On lowering the pH below the pKa of the phosphate groups the amount of bound Mg2+ decreased concomitantly with decreasing ionization of the phosphate groups. Both the amount of Mg2+ bound to the teichoic acid and the apparent association constants were similar in the presence of 10 mM concentrations of NaCl or KCl but decreased markedly in the presence of 10 mM-CaCl2 because of competition between Ca2+ and Mg2+ for the binding sites. A similar effect was found when the concentration of NaCl was increased from 0 to 50 mM. The results are discussed in relation to the function of teichoic acid in the walls of Gram-positive bacteria.
摘要
在可控离子浓度和pH条件下，通过平衡透析法测定了Mg2+与布氏乳杆菌N.C.I.B. 8007细胞壁磷壁酸的结合情况。在pH 5.0、含有10mM-NaCl的水溶液中，每两个磷壁酸磷酸基团结合一个Mg2+离子，表观缔合常数Kassoc. = 2.7×10(3) M-1。当pH降至磷酸基团的pKa以下时，结合的Mg2+量随着磷酸基团电离程度的降低而相应减少。在10 mM浓度的NaCl或KCl存在下，与磷壁酸结合的Mg2+量和表观缔合常数相似，但在10 mM-CaCl2存在下，由于Ca2+和Mg2+对结合位点的竞争，它们显著降低。当NaCl浓度从0增加到50 mM时，也发现了类似的效应。结合革兰氏阳性菌细胞壁中磷壁酸的功能对结果进行了讨论。
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