阿糖胞苷蛋白的体内滴定
In vivo titration of araC protein.
作者信息
Haggerty D M, Oeschger M P, Schleif R F
出版信息
J Bacteriol. 1978 Sep;135(3):775-81. doi: 10.1128/jb.135.3.775-781.1978.
Abstract
The requirement for araC protein in the induction of the araBAD operon was investigated. Strains of Escherichia coli carrying an araC(Am) mutation and temperature-sensitive amber suppressors were used to vary the intracellular level of araC protein. The levels of araC protein studied ranged from 0.007 to 1.8 times the normal amount. The results indicate that the normal level of araC protein is just sufficient to provide maximal expression of the araBAD operon.
摘要
研究了阿拉伯糖操纵子诱导过程中对阿拉伯糖C蛋白的需求。使用携带阿拉伯糖C（Am）突变和温度敏感型琥珀抑制子的大肠杆菌菌株来改变细胞内阿拉伯糖C蛋白的水平。所研究的阿拉伯糖C蛋白水平范围为正常量的0.007至1.8倍。结果表明，阿拉伯糖C蛋白的正常水平刚好足以使阿拉伯糖操纵子实现最大表达。
相似文献
1
In vivo titration of araC protein.阿糖胞苷蛋白的体内滴定
J Bacteriol. 1978 Sep;135(3):775-81. doi: 10.1128/jb.135.3.775-781.1978.
2
Dominance relationships among mutant alleles of regulatory gene araC in the Escherichia coli B/R L-arabinose operon.大肠杆菌B/R L-阿拉伯糖操纵子中调控基因araC突变等位基因之间的显性关系。
J Bacteriol. 1986 Nov;168(2):999-1001. doi: 10.1128/jb.168.2.999-1001.1986.
3
Positively regulated expression of the Escherichia coli araBAD promoter in Corynebacterium glutamicum.大肠杆菌araBAD启动子在谷氨酸棒杆菌中的正调控表达
FEMS Microbiol Lett. 1999 May 1;174(1):125-30. doi: 10.1111/j.1574-6968.1999.tb13558.x.
4
Suppressed nonsense mutations in the araC gene of Escherichia coli provide three novel variant proteins.大肠杆菌araC基因中被抑制的无义突变产生了三种新型变体蛋白。
J Bacteriol. 1983 Jun;154(3):1329-38. doi: 10.1128/jb.154.3.1329-1338.1983.
5
Cyclic adenosine 3',5'-monophosphate-mediated hyperinduction of araBAD and lacZYA expression in a crp mutant of Escherichia coli K-12.环磷酸腺苷介导的大肠杆菌K-12 crp突变体中araBAD和lacZYA表达的超诱导
J Bacteriol. 1981 Aug;147(2):500-8. doi: 10.1128/jb.147.2.500-508.1981.
6
In vitro activation of the transcription of araBAD operon by araC activator.araC激活剂对araBAD操纵子转录的体外激活作用。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):634-8. doi: 10.1073/pnas.71.3.634.
7
Constitutive mutations in the controlling site region of the araBAD operon of Escherichia coli B/r that decrease sensitivity to catabolite repression.大肠杆菌B/r的araBAD操纵子控制位点区域中的组成型突变，降低了对分解代谢物阻遏的敏感性。
J Bacteriol. 1977 Feb;129(2):948-58. doi: 10.1128/jb.129.2.948-958.1977.
8
Mutations affecting catabolite repression of the L-arabinose regulon in Escherichia coli B/r.影响大肠杆菌B/r中L-阿拉伯糖操纵子分解代谢阻遏的突变
J Bacteriol. 1976 Jun;126(3):1119-31. doi: 10.1128/jb.126.3.1119-1131.1976.
9
Apo-AraC actively seeks to loop.阿糖胞苷积极寻求环化。
J Mol Biol. 1998 May 8;278(3):529-38. doi: 10.1006/jmbi.1998.1713.
10
The araC promoter: transcription, mapping and interaction with the araBAD promoter.阿拉伯糖操纵子C基因启动子：转录、图谱绘制以及与阿拉伯糖操纵子BAD基因启动子的相互作用
Cell. 1977 Jul;11(3):545-50. doi: 10.1016/0092-8674(77)90072-1.
引用本文的文献
1
Mutations to constitutivity and derepression are separate and separable in a regulatory gene of Aspergillus nidulans.在 Aspergillus nidulans 的一个调控基因中，组成型和去阻遏突变是分开的，也是可分离的。
Curr Genet. 1981 Sep;4(1):63-8. doi: 10.1007/BF00376787.
2
Integration of transcriptional inputs at promoters of the arabinose catabolic pathway.阿拉伯糖分解代谢途径启动子处转录输入的整合
BMC Syst Biol. 2010 Jun 2;4:75. doi: 10.1186/1752-0509-4-75.
3
Use of an in vivo titration method to study a global regulator: effect of varying Lrp levels on expression of gltBDF in Escherichia coli.使用体内滴定法研究全局调控因子：改变Lrp水平对大肠杆菌中gltBDF表达的影响。
J Bacteriol. 1996 Dec;178(23):6904-12. doi: 10.1128/jb.178.23.6904-6912.1996.
4
High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: isolation of strains with suppressor-enhancing mutations.大肠杆菌K12的高效温度敏感琥珀抑制菌株：具有抑制增强突变的菌株的分离
Mol Gen Genet. 1980;177(4):545-52. doi: 10.1007/BF00272662.
5
High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: construction and characterization of recombinant strains with suppressor-enhancing mutations.大肠杆菌K12的高效温度敏感琥珀抑制菌株：具有抑制增强突变的重组菌株的构建与表征
Mol Gen Genet. 1980;178(2):293-9. doi: 10.1007/BF00270475.
6
The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.大肠杆菌L-阿拉伯糖操纵子：调节蛋白的结合位点及正负调控机制
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3346-50. doi: 10.1073/pnas.77.6.3346.
本文引用的文献
1
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
2
Adjacent effect on suppression efficiency. II. Study on ochre and amber mutants of T4 phage lysozyme.抑制效率的相邻效应。II. T4噬菌体溶菌酶赭石型和琥珀型突变体的研究。
Mol Gen Genet. 1970;106(3):208-12. doi: 10.1007/BF00340380.
3
Complementation studies in the maltose-A region of the Escherichia coli K12 genetic map.大肠杆菌K12遗传图谱中麦芽糖-A区域的互补研究。
J Mol Biol. 1971 Nov 14;61(3):681-94. doi: 10.1016/0022-2836(71)90072-6.
4
An L-arabinose binding protein and arabinose permeation in Escherichia coli.大肠杆菌中的一种L-阿拉伯糖结合蛋白与阿拉伯糖渗透作用
J Mol Biol. 1969 Nov 28;46(1):185-96. doi: 10.1016/0022-2836(69)90065-5.
5
The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene.大肠杆菌B-r中的L-阿拉伯糖操纵子：调节基因产物两种功能状态的遗传学证明。
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1100-7. doi: 10.1073/pnas.62.4.1100.
6
Arabinose-leucine deletion mutants of Escherichia coli B-r.大肠杆菌B-r的阿拉伯糖-亮氨酸缺失突变体
J Bacteriol. 1969 Jun;98(3):1159-69. doi: 10.1128/jb.98.3.1159-1169.1969.
7
In vivo experiments on the mechanism of action of L-arabinose C gene activator and lactose repressor.L-阿拉伯糖C基因激活剂和乳糖阻遏物作用机制的体内实验
J Mol Biol. 1973 Nov 5;80(3):433-44. doi: 10.1016/0022-2836(73)90414-2.
8
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.
9
Fine-structure deletion map of the Escherichia coli L-arabinose operon.大肠杆菌L-阿拉伯糖操纵子的精细结构缺失图谱。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3479-84. doi: 10.1073/pnas.69.11.3479.
10
Regulation of the L-arabinose operon BAD in vitro.体外对L-阿拉伯糖操纵子BAD的调控
J Biol Chem. 1974 May 10;249(9):2946-52.
Zotero 插件