Imai M, Hamada C, Uetake H
Jpn J Microbiol. 1976 Oct;20(5):455-64. doi: 10.1111/j.1348-0421.1976.tb01011.x.
The possibility that virus multiplication may be inhibited by interaction of infected cells with uninfected cells was tested by experiments, using human adenovirus type 12 (Ad12). Permissive human cells (human embryonic kidney-HEK,KB or HeLa) were infected and seeded on uninfected or infected "nonpermissive" cell (human embryonic lung=HEL) monolayers, and virus yields or proportions of viral antigen-synthesizing cells were compared with each other. Both the virus yields and the proportions of viral antigen-positive cells were not reduced significantly when seeded on infected HEL cells, while they seeded on uninfected HEL cells both of them were reduced remarkably, compared with the yield and the proportion of controls seeded on glass. Similar results were obtained regardless of the type of permissive cells, HEK, KB, or HeLa. Similar reduction of the yield was observed when seeded on HEL cells infected with Ad12 inactivated by heat or by antiserum, and partial reduction was observed when seeded on HEL cells infected with UV-inactivated Ad12, depending on the extent of UV dosis. These experiments showed that intracellular virus multiplication may be inhibited by interaction of infected cells with uninfected cells, and this may be due to the difference in the cell surface structure.
利用人12型腺病毒（Ad12）通过实验测试了感染细胞与未感染细胞相互作用抑制病毒增殖的可能性。将允许性人细胞（人胚肾细胞 - HEK、KB或HeLa）进行感染，然后接种到未感染或感染的“非允许性”细胞（人胚肺细胞 - HEL）单层上，并相互比较病毒产量或病毒抗原合成细胞的比例。当接种到感染的HEL细胞上时，病毒产量和病毒抗原阳性细胞的比例均未显著降低，而当接种到未感染的HEL细胞上时，与接种在玻璃上的对照的产量和比例相比，二者均显著降低。无论允许性细胞类型是HEK、KB还是HeLa，均得到相似结果。当接种到经热或抗血清灭活的Ad12感染的HEL细胞上时，观察到产量有类似降低，而当接种到经紫外线灭活的Ad12感染的HEL细胞上时，根据紫外线剂量的程度观察到部分降低。这些实验表明，感染细胞与未感染细胞的相互作用可能抑制细胞内病毒增殖，这可能是由于细胞表面结构的差异所致。
