Bourne K A, Major E O, Khoobyarian N
In Vitro. 1979 Sep;15(9):664-8. doi: 10.1007/BF02618244.
Cell transformation induced by murine sarcoma virus (MSV-M) is significantly inhibited (80--90%) in a clonal line of normal rat kidney (NRK) cells when they are infected with rat cell passaged adenovirus 12 (R-Ad12). No inhibition is seen when R-Ad12 is added simultaneously with, or 1 1/2 or 24 hr after, MSV-M infection, suggesting that inhibition occurs most probably intracellularly. There is also a direct correlation between the extent of focus formation and the concentration of R-Ad12 used. Concentrations of R-Ad12 used to inhibit cell transformation do not affect cell growth. Significant inhibition in foci (90--100%) is also evident in the first two subcultures of R-Ad12 infected NRK cells. The mechanism of the inhibition is not yet known.
当正常大鼠肾（NRK）细胞的克隆系被大鼠细胞传代的腺病毒12（R-Ad12）感染时，鼠肉瘤病毒（MSV-M）诱导的细胞转化受到显著抑制（80%-90%）。当R-Ad12在MSV-M感染的同时、感染后1.5小时或24小时添加时，未观察到抑制作用，这表明抑制作用很可能发生在细胞内。病灶形成的程度与所用R-Ad12的浓度之间也存在直接相关性。用于抑制细胞转化的R-Ad12浓度不影响细胞生长。在R-Ad12感染的NRK细胞的前两代传代培养中，病灶的显著抑制（90%-100%）也很明显。抑制机制尚不清楚。
