Sorg C, Geczy C L
J Immunol. 1978 Sep;121(3):1199-205.
It was shown previously, that an antiserum directed against highly purified fractions of migration inhibitory factor inhibits delayed hypersensitivity reactions in vivo and in vitro. Using radiolabeling techniques we determined that the anti-lymphokine serum reacted primarily with three lymphocyte activation products (m.w. 60,000, 45,000, and 30,000) all of which had a similar isoelectric point of 5.2. The cellular origin of this material was investigated. It was found that activated B cells, B leukemia cells (L2C), and growing fibroblasts produced material of a similar m.w. as analyzed on SDS-PAGE. No cross-reaction was found with radiolabeled products of activated murine and human lymph node cells and of SV 40-infected African green monkey kidney cells. The isoelectric point of the reactive material from B cells, leukemia cells, and fibroblasts was determined at 5.2. In addition to material with pI 5.2, lymph node cells also produced material with pI 3.5 to 4.5, which focused at pH 5.0 to 5.4. After neuraminidase treatment macrophage migration inhibitory activity in fibroblast culture supernatants could be absorbed specifically to insolubilized anti-lymphokine antibody. These findings suggests that lymphoid and nonlymphoid cells are capable of producing molecules whose physicochemical and functional properties appear to be identical.
先前已表明，针对高度纯化的迁移抑制因子组分的抗血清在体内和体外均能抑制迟发型超敏反应。利用放射性标记技术，我们确定抗淋巴因子血清主要与三种淋巴细胞活化产物（分子量分别为60,000、45,000和30,000）发生反应，所有这些产物的等电点均相似，为5.2。我们对这种物质的细胞来源进行了研究。结果发现，活化的B细胞、B白血病细胞（L2C）和成纤维细胞产生的物质分子量相似，经十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析得出此结果。在活化的小鼠和人淋巴结细胞以及感染SV 40的非洲绿猴肾细胞的放射性标记产物中未发现交叉反应。B细胞、白血病细胞和成纤维细胞中反应性物质的等电点测定为5.2。除了等电点为5.2的物质外，淋巴结细胞还产生等电点为3.5至4.5的物质，该物质在pH 5.0至5.4聚焦。经神经氨酸酶处理后，成纤维细胞培养上清液中的巨噬细胞迁移抑制活性可被特异性吸附到不溶性抗淋巴因子抗体上。这些发现表明，淋巴细胞和非淋巴细胞能够产生理化性质和功能特性似乎相同的分子。
