Nassar A, Morshedi M, Mahony M, Srisombut C, Lin M H, Oehninger S
Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23507, USA.
Andrologia. 1999 Jan;31(1):9-15. doi: 10.1046/j.1439-0272.1999.00241.x.
Pentoxifylline (PTX) was incubated in vitro with human spermatozoa to examine its effects on sperm motility characteristics and bovine cervical mucus penetrability (BCMP). Sperm motion parameters were assessed by computer-assisted motion analysis (CASA) using HTM-IVOS and BCMP was evaluated using the Penetrak kit. In vitro incubation with PTX (1 mg ml-1; 3.6 mM, 30 min) did not significantly change percentage motility, average path velocity (VAP), straight-line velocity (VSL) or beat cross frequency (BCF) of spermatozoa from normozoospermic or asthenozoospermic samples. However, it significantly increased curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and hyperactivated motility (HA), and significantly decreased linearity (LIN) of spermatozoa from both samples. Pentoxifylline was found to increase BCMP scores for spermatozoa from asthenozoospermic samples, but did not affect scores for spermatozoa from normozoospermic samples. Bovine cervical mucus penetrability (BCMP) was found to be positively and significantly correlated with the percentage motility of both non-PTX-treated and PTX-treated spermatozoa for asthenozoospermic samples. These results demonstrated that PTX enhanced several motion sperm parameters as well as BCMP in asthenozoospermic samples and suggest a potential use of the methylxanthine in infertile patients with motility defects undergoing artificial insemination.
己酮可可碱（PTX）与人精子在体外进行孵育，以研究其对精子运动特征和牛宫颈黏液穿透性（BCMP）的影响。精子运动参数通过使用HTM-IVOS的计算机辅助运动分析（CASA）进行评估，BCMP则使用Penetrak试剂盒进行评估。用PTX（1mg/ml；3.6mM，30分钟）进行体外孵育，对正常精子样本或弱精子症样本中的精子活力百分比、平均路径速度（VAP）、直线速度（VSL）或鞭打交叉频率（BCF）没有显著影响。然而，它显著增加了两个样本中精子的曲线速度（VCL）、头部侧向位移幅度（ALH）和超激活运动（HA），并显著降低了精子的线性度（LIN）。己酮可可碱可提高弱精子症样本中精子的BCMP评分，但对正常精子症样本中的精子评分没有影响。对于弱精子症样本，发现牛宫颈黏液穿透性（BCMP）与未用PTX处理和用PTX处理的精子的活力百分比呈正相关且显著相关。这些结果表明，PTX增强了弱精子症样本中的几个精子运动参数以及BCMP，并提示甲基黄嘌呤在接受人工授精的有运动缺陷的不育患者中具有潜在用途。
