Raschke M J, Bail H, Windhagen H J, Kolbeck S F, Weiler A, Raun K, Kappelgard A, Skiaerbaek C, Haas N P
Trauma and Reconstructive Surgery, Medical Faculty Charité, Virchow Clinic, Humboldt University of Berlin, Germany.
Bone. 1999 Feb;24(2):81-8. doi: 10.1016/s8756-3282(98)00158-6.
The purpose of the present study was to prove whether homologous GH has a stimulating effect on bone healing. Therefore, left tibiae of 30 micropigs were osteomized and distracted over an external fixator at the rate of 2 mm/day on each of 10 consecutive days. Animals were killed after a healing period of another 10 days. The treatment group received 100 microg of recombinant porcine growth hormone (rpGH) per kilogram of body weight per day. Serial torsional nondestructive biomechanical tests were performed in vivo using a newly developed measurement device. After killing, destructive torsional strength testing of the sites of distraction was performed. To determine the endocrine response to the administration of rpGH, serum levels of insulin-like growth factor-I (IGF-I) were determined. Nondestructive in vivo testing showed that torsional stiffness of the regenerate was significantly higher in the treatment group than in the control group. Final regenerate torsional failure load was 131% higher and ultimate torsional stiffness was 231% higher in the treatment group than in the control group. The mean serum level of IGF-I increased to 440% of preoperative basal level in the treatment group and remained unchanged in the control group. Our data indicate that systemic administration of recombinant homologous growth hormone greatly accelerates ossification of bone regenerate in distraction osteogenesis.
本研究的目的是证明同源生长激素（GH）是否对骨愈合有刺激作用。因此，选取30只小型猪的左胫骨进行截骨，并通过外固定器以每天2毫米的速度连续牵引10天。在再过10天的愈合期后处死动物。治疗组每天每千克体重接受100微克重组猪生长激素（rpGH）。使用新开发的测量装置在体内进行连续的扭转非破坏性生物力学测试。处死动物后，对牵引部位进行破坏性扭转强度测试。为了确定对rpGH给药的内分泌反应，测定血清胰岛素样生长因子-I（IGF-I）水平。体内非破坏性测试表明，治疗组再生骨的扭转刚度明显高于对照组。治疗组最终再生骨扭转破坏载荷比对照组高131%，极限扭转刚度比对照组高231%。治疗组IGF-I的平均血清水平升至术前基础水平的440%，而对照组保持不变。我们的数据表明，全身给予重组同源生长激素可大大加速牵张成骨中骨再生的骨化。
