Kull K, Salupere R, Uibo R, Ots M, Salupere V
Department of Internal Medicine, University of Tartu, Estonia.
Hepatogastroenterology. 1998 Nov-Dec;45(24):2132-7.
BACKGROUND/AIMS: In Estonia, the incidence of ulcerative colitis and especially Crohn's disease appears to be rare. Antineutrophil cytoplasmic antibodies (ANCA) are frequently found in ulcerative colitis but less frequently in Crohn's disease, their pathophysiological significance is still unclear.
Fifty-nine serum samples from patients with ulcerative colitis, 17 with Crohn's disease, 25 with irritable bowel syndrome, and 86 healthy persons were studied. Sera were analyzed for the presence of ANCA by indirect immunofluorescence, and enzyme-linked immunosorbent assay for specific ANCA using different antigens was performed.
ANCA were detected in 29 of 59 (49%) patients with ulcerative colitis, 4 of 17 (24%) patients with Crohn's disease, and in 4 of 111 (4%) controls. The immunofluorescence staining was mostly perinuclear (pANCA). There was no correlation between ANCA and the duration or extent of the inflammatory bowel disease. In specific enzyme-linked immunosorbent assays, only 14 sera elicited binding above the normal range.
Although the prevalence of ulcerative colitis and Crohn's disease in Estonia is much lower than in European countries, there seem to be no differences in the presence of ANCA.
背景/目的：在爱沙尼亚，溃疡性结肠炎尤其是克罗恩病的发病率似乎较低。抗中性粒细胞胞浆抗体（ANCA）在溃疡性结肠炎中经常被发现，但在克罗恩病中较少见，其病理生理意义仍不清楚。
研究了59例溃疡性结肠炎患者、17例克罗恩病患者、25例肠易激综合征患者和86名健康人的血清样本。通过间接免疫荧光分析血清中ANCA的存在情况，并使用不同抗原进行特异性ANCA的酶联免疫吸附测定。
59例溃疡性结肠炎患者中有29例（49%）检测到ANCA，17例克罗恩病患者中有4例（24%）检测到ANCA，111名对照中有4例（4%）检测到ANCA。免疫荧光染色大多为核周型（pANCA）。ANCA与炎症性肠病的病程或范围之间无相关性。在特异性酶联免疫吸附测定中，只有14份血清的结合高于正常范围。
尽管爱沙尼亚溃疡性结肠炎和克罗恩病的患病率远低于欧洲国家，但ANCA的存在情况似乎没有差异。
