应激对禁食大鼠不同组织中氨基酸及相关化合物的影响。

Effects of stress on amino acids and related compounds in various tissues of fasted rats.

作者信息

Dadmarz M, vd Burg C, Milakofsky L, Hofford J M, Vogel W H

机构信息

Department of Biochemistry, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107, USA.

出版信息

Life Sci. 1998;63(16):1485-91. doi: 10.1016/s0024-3205(98)00415-9.

DOI:10.1016/s0024-3205(98)00415-9

PMID:9952294

Abstract

The purpose of this study was to examine the effect of stress on the free amino acid pattern of plasma and various organs. Two groups of rats were deprived of food, for 24 hrs. One group was sacrificed after this time (fasting control representing mostly free endogenous amino acids) and the second group was first restrained in wire cages for 120 min before being sacrificed (fasting stress representing mostly the effects of stress on endogenous free amino acids). A third group had free access to food and was sacrificed at the same time (fed control representing mostly free amino acids absorbed from the gut and endogenous free amino acid metabolism). Fasting (as compared to fed controls) reduced alanine and arginine but increased ethanolamine, glutamic acid and glutamine in the plasma; increased ethanolamine, phosphoethanolamine and glutamic acid in the liver; increased carnosine, glutamic acid, phosphoethanolamine and glutamine in the ventricle; increased oxidized glutathione in the aorta; decreased alanine, aspartic acid, glutamic acid, leucine and methionine and increased glutamine in the pancreas; and decreased arginine in skeletal muscle. Fasting plus stress (as compared to fasting controls) reduced alanine and glutamine in the plasma; increased methionine in the liver; increased ethanolamine, GABA, and glutamic acid in the aorta; reduced arginine, glutamic acid, glutamine, leucine and methionine but increased ethanolamine in the ventricle; reduced ammonia and ethanolamine but increased histidine, isoleucine, leucine, lysine, phenylalanine, tyrosine and valine in the pancreas; and reduced ammonia in skeletal muscle. Fasting plus stress affects the amino acid composition of plasma and various of tissues but effects seen were individually different and strongly substance and tissue specific. Plasma changes did not coincide with tissue changes. Changes in the endogenous pattern of amino acids and related compounds in response to stress could be first indications of stress induced organ pathology.

摘要

本研究的目的是检测应激对血浆及各器官游离氨基酸模式的影响。将两组大鼠禁食24小时。一组在这段时间后处死（禁食对照组，主要代表内源性游离氨基酸），另一组在处死前先在铁丝笼中束缚120分钟（禁食应激组，主要代表应激对内源性游离氨基酸的影响）。第三组可自由进食，并在同一时间处死（喂食对照组，主要代表从肠道吸收的游离氨基酸及内源性游离氨基酸代谢）。与喂食对照组相比，禁食使血浆中的丙氨酸和精氨酸减少，但乙醇胺、谷氨酸和谷氨酰胺增加；使肝脏中的乙醇胺、磷酸乙醇胺和谷氨酸增加；使心室中的肌肽、谷氨酸、磷酸乙醇胺和谷氨酰胺增加；使主动脉中的氧化型谷胱甘肽增加；使胰腺中的丙氨酸、天冬氨酸、谷氨酸、亮氨酸和蛋氨酸减少，谷氨酰胺增加；使骨骼肌中的精氨酸减少。与禁食对照组相比，禁食加应激使血浆中的丙氨酸和谷氨酰胺减少；使肝脏中的蛋氨酸增加；使主动脉中的乙醇胺、γ-氨基丁酸和谷氨酸增加；使心室中的精氨酸、谷氨酸、谷氨酰胺、亮氨酸和蛋氨酸减少，但乙醇胺增加；使胰腺中的氨和乙醇胺减少，但组氨酸、异亮氨酸、亮氨酸、赖氨酸、苯丙氨酸、酪氨酸和缬氨酸增加；使骨骼肌中的氨减少。禁食加应激影响血浆和各种组织的氨基酸组成，但观察到的影响因物质和组织而异。血浆变化与组织变化不一致。应激引起的氨基酸及相关化合物内源性模式的变化可能是应激诱导器官病变的早期迹象。