种间DNA/DNA杂交揭示小麦基因组中不同重复序列的散布情况。
Interspersion of different repeated sequences in the wheat genome revealed by interspecies DNA/DNA hybridisation.
作者信息
Smith D B, Rimpau J, Flavell R B
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2811-25. doi: 10.1093/nar/3.10.2811.
The repeated sequences in oats DNA have been used to study chromosomal repeated sequence organisation in wheat. Approximately 75% of the wheat genome consists of repeated sequences but only approximately 20% will form heteroduplexes with repeated sequences from oats DNA at 60 degrees C in 0.18 M Na+. The proportion of wheat DNA that forms heteroduplexes with oats DNA is shown to be independent of the wheat DNA fragment length. However, the proportion of wheat DNA that is retained with the heteroduplexes when fractionated on hydroxyapatite is very dependent upon the wheat fragment length up to 3500 nucleotides. This is because more non-renatured wheat DNA is attached to the heteroduplexes with longer fragments. The results indicate that the repeated sequences in the wheat genome homologous to repeated sequences in oats are not clustered in the chromosomes but distributed amongst other repeated and possible non-repeated sequences.
燕麦DNA中的重复序列已被用于研究小麦染色体重复序列的组织。小麦基因组中约75%由重复序列组成，但在0.18M Na+中于60℃时，只有约20%会与燕麦DNA的重复序列形成异源双链体。与燕麦DNA形成异源双链体的小麦DNA比例与小麦DNA片段长度无关。然而，当在羟基磷灰石上进行分级分离时，与异源双链体保留在一起的小麦DNA比例在长达3500个核苷酸的范围内非常依赖于小麦片段长度。这是因为更多未复性的小麦DNA与较长片段的异源双链体相连。结果表明，小麦基因组中与燕麦重复序列同源的重复序列并非聚集在染色体上，而是分布在其他重复序列以及可能的非重复序列之中。
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