Leber H W, Gleumes L, Schütterle G
Kidney Int Suppl. 1978 Jun(8):S43-8.
Forty-five days after subtotal nephrectomy or sham-operation of male rats, microsomal enzymes were investigated in vitro. The activities (per milligram) of microsomal protein of two esterases and of two glucuronyltransferases were normal in the uremic rats. The mixed-function oxidation system had lower activities per milligram of protein than that in sham-operated controls. Due to a decrease of the microsomal protein content of the uremic liver, the activities of these enzymes were decreased when calculated for the whole liver. In contrast, the glucoronidation of phenolphthalein remained normal when related to the whole liver, due to an increased activity per mg of protein. Treatment with the plasticizer di-(2-ethylhexyl)-phthalate caused a significant increase of the liver wet weight, the microsomal protein content, and the activity per mg of protein for the demethylation of aminopyrine in subtotally nephrectomized rats but was without influence on the liver of sham-operated controls. It is concluded that uremia itself does not induce liver microsomal enzymes. The microsomal enzymes, however, remain inducible by foreign compounds even under uremic conditions.
雄性大鼠进行肾次全切除或假手术后45天，对微粒体酶进行体外研究。在尿毒症大鼠中，两种酯酶和两种葡萄糖醛酸转移酶的微粒体蛋白活性（每毫克）正常。混合功能氧化系统每毫克蛋白的活性低于假手术对照组。由于尿毒症肝脏微粒体蛋白含量降低，按全肝计算时这些酶的活性降低。相比之下，酚酞的葡萄糖醛酸化与全肝相关时仍保持正常，因为每毫克蛋白的活性增加。用增塑剂邻苯二甲酸二（2-乙基己基）酯处理导致肾次全切除大鼠的肝脏湿重、微粒体蛋白含量以及氨基比林去甲基化每毫克蛋白的活性显著增加，但对假手术对照组的肝脏无影响。结论是尿毒症本身不会诱导肝脏微粒体酶。然而，即使在尿毒症条件下，微粒体酶仍可被外来化合物诱导。
