Lindemann R, Laerum O D
Scand J Haematol. 1976 Oct;17(4):293-9.
The specificity and toxicity of the urinary erythropoiesis inhibiting factor (EIF) has been tested both in vivo and in vitro. When EIF was given to ESF stimulated erythropoietically suppressed polycythaemic mice and to mice at maximal endogenous erythropoietic stimulation, a reduction of the erythroid bone marrow cells, the erythropoietic 3H-TdR L.I. and the total number of bone marrow cells were observed. No effect was seen on the myelopoietic bone marrow cells. An unspecific toxic effect was unlikely, since addition of EIF did not alter the proliferation of lymphoblastic cells nor change the glucose utilization of bone marrow cells in vitro. Neither did the amount of dead bone marrow cells increase after being incubated with EIF for 72 h. The results indicate that the urinary EIF is a non-toxic, cell specific inhibitor on erythropoiesis.
已在体内和体外对尿红细胞生成抑制因子（EIF）的特异性和毒性进行了测试。当将EIF给予促红细胞生成素刺激的红细胞生成受抑制的多血症小鼠以及处于最大内源性红细胞生成刺激状态的小鼠时，观察到红系骨髓细胞、红细胞生成的3H-胸腺嘧啶核苷标记指数（L.I.）和骨髓细胞总数减少。对粒系骨髓细胞未见影响。不太可能存在非特异性毒性作用，因为添加EIF不会改变体外淋巴细胞的增殖，也不会改变骨髓细胞的葡萄糖利用。用EIF孵育72小时后，死骨髓细胞的数量也没有增加。结果表明，尿EIF是一种对红细胞生成无毒的细胞特异性抑制剂。
