Staples J W, Fritz G E
Transfusion. 1976 Nov-Dec;16(6):566-70. doi: 10.1046/j.1537-2995.1976.16677060240.x.
Repeated transfusion of small increments of blood are frequently required for the sick and premature newborn infant to correct endogenous hypovolemia and/or to replace blood obtained for laboratory monitoring purposes. Previously fresh group and type specific whole blood was used. To eliminate waste of fresh whole blood, maintain fresh red blood cell properties, eliminate the hazards of transfusing plasma and to provide a more efficient system, a pediatric frozen red cell pack (PFRCP) has been developed. Units of group O rr red blood cells are glycerolized using a high glycerol method. The glycerolized red blood cells are separated into three equal aliquots and frozen. When needed, the PFRCP are deglycerolized by a modified procedure using the IBM Cell Processor. During a six month period, 71 infants were given 153 separate transfusions of deglycerolized red blood cells using 102 PFRCP prepared from 34 units of red blood cells. Red blood cell recovery, hematocrit, white blood cell removal, residual glycerol, total protein, and supernatant hemoglobin levels were measured. Clinical response was followed and found to be excellent.
患病和早产的新生儿经常需要反复少量输血，以纠正内源性血容量不足和/或补充为实验室监测目的而采集的血液。以前使用的是新鲜的同型和同血型全血。为了消除新鲜全血的浪费，保持新鲜红细胞的特性，消除输注血浆的风险，并提供一个更有效的系统，已经开发了一种儿科冷冻红细胞包（PFRCP）。O型rr红细胞单位采用高甘油法进行甘油化处理。甘油化红细胞被分成三个相等的等分并冷冻。需要时，使用IBM细胞处理器通过改良程序对PFRCP进行去甘油化处理。在六个月的时间里，71名婴儿接受了153次单独的去甘油化红细胞输血，使用了由34个红细胞单位制备的102个PFRCP。测量了红细胞回收率、血细胞比容、白细胞去除率、残余甘油、总蛋白和上清血红蛋白水平。跟踪临床反应，发现效果极佳。
