裸鼠模型中人前列腺癌最佳冷冻参数的测定
Determination of optimal freezing parameters of human prostate cancer in a nude mouse model.
作者信息
Turk T M, Rees M A, Pietrow P, Myers C E, Mills S E, Gillenwater J Y
机构信息
Department of Urology, University of Virginia Health Sciences Center, Charlottesville, USA.
出版信息
Prostate. 1999 Feb 1;38(2):137-43. doi: 10.1002/(sici)1097-0045(19990201)38:2<137::aid-pros7>3.0.co;2-5.
BACKGROUND
We sought to determine whether more than one freeze/thaw cycle is required and what minimum temperature reliably kills prostate cancer in vivo.
METHODS
Two human prostate cancer cell lines (LNCaP and PC3) were implanted subcutaneously in male nude BALB/c mice. Tumors were frozen with contemporary cryosurgery equipment and monitored for temperature, size, and serum prostate-specific antigen (PSA) measurements. The tumors were subjected to one or two freeze/thaw cycles through a wide range of temperatures from 0 - -80 degrees C.
RESULTS
These experiments show that a single freeze/thaw to a temperature < -40 degrees C is adequate to kill most tumor cells in this mouse model of prostate cancer.
CONCLUSIONS
Freezing prostate cancer to < -40 degrees C and ensuring that the entire tumor is frozen is more important than additional freeze/thaw cycles in this experimental model.
背景
我们试图确定是否需要不止一个冻融循环，以及在体内可靠杀死前列腺癌的最低温度是多少。
方法
将两个人类前列腺癌细胞系（LNCaP和PC3）皮下植入雄性裸BALB/c小鼠体内。用当代冷冻手术设备对肿瘤进行冷冻，并监测温度、大小和血清前列腺特异性抗原（PSA）测量值。肿瘤在0至-80摄氏度的广泛温度范围内经历一或两个冻融循环。
结果
这些实验表明，在这个前列腺癌小鼠模型中，单次冻融至温度<-40摄氏度足以杀死大多数肿瘤细胞。
结论
在这个实验模型中，将前列腺癌冷冻至<-40摄氏度并确保整个肿瘤被冷冻比额外的冻融循环更重要。
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