Turk T M, Rees M A, Pietrow P, Myers C E, Mills S E, Gillenwater J Y
Department of Urology, University of Virginia Health Sciences Center, Charlottesville, USA.
Prostate. 1999 Feb 1;38(2):137-43. doi: 10.1002/(sici)1097-0045(19990201)38:2<137::aid-pros7>3.0.co;2-5.
We sought to determine whether more than one freeze/thaw cycle is required and what minimum temperature reliably kills prostate cancer in vivo.
Two human prostate cancer cell lines (LNCaP and PC3) were implanted subcutaneously in male nude BALB/c mice. Tumors were frozen with contemporary cryosurgery equipment and monitored for temperature, size, and serum prostate-specific antigen (PSA) measurements. The tumors were subjected to one or two freeze/thaw cycles through a wide range of temperatures from 0 - -80 degrees C.
These experiments show that a single freeze/thaw to a temperature < -40 degrees C is adequate to kill most tumor cells in this mouse model of prostate cancer.
Freezing prostate cancer to < -40 degrees C and ensuring that the entire tumor is frozen is more important than additional freeze/thaw cycles in this experimental model.
我们试图确定是否需要不止一个冻融循环，以及在体内可靠杀死前列腺癌的最低温度是多少。
将两个人类前列腺癌细胞系（LNCaP和PC3）皮下植入雄性裸BALB/c小鼠体内。用当代冷冻手术设备对肿瘤进行冷冻，并监测温度、大小和血清前列腺特异性抗原（PSA）测量值。肿瘤在0至-80摄氏度的广泛温度范围内经历一或两个冻融循环。
这些实验表明，在这个前列腺癌小鼠模型中，单次冻融至温度<-40摄氏度足以杀死大多数肿瘤细胞。
在这个实验模型中，将前列腺癌冷冻至<-40摄氏度并确保整个肿瘤被冷冻比额外的冻融循环更重要。
