携带与thyA共转导的rec突变的大肠杆菌K-12重组缺陷型突变体的遗传分析。
Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA.
作者信息
Willetts N S, Mount D W
出版信息
J Bacteriol. 1969 Nov;100(2):923-34. doi: 10.1128/jb.100.2.923-934.1969.
Abstract
The rec mutations carried by 20 strains of Escherichia coli K-12 which are defective in genetic recombination and sensitive to ultraviolet light and X rays, and whose lambda lysogens show spontaneous phage production, have been mapped near thyA. In 15 of the strains, the rec mutation fails to complement recB21 but complements rec-22. The other five strains carry a rec mutation which complements recB21 but not rec-22. These mutations map closer to thyA than those which fail to complement recB21. They therefore appear to be defective in a different recombination gene, denoted recC. The order of recB and recC on the linkage map of E. coli K-12 is thyA-recC-recB-argA.
摘要
20株大肠杆菌K - 12携带的rec突变已被定位在thyA附近。这些菌株在基因重组方面存在缺陷，对紫外线和X射线敏感，其λ溶原菌表现出自发噬菌体产生。在15株菌株中，rec突变不能与recB21互补，但能与rec - 22互补。另外5株菌株携带的rec突变能与recB21互补，但不能与rec - 22互补。这些突变在连锁图谱上比那些不能与recB21互补的突变更靠近thyA。因此，它们似乎在一个不同的重组基因中存在缺陷，该基因被命名为recC。在大肠杆菌K - 12的连锁图谱上，recB和recC的顺序是thyA - recC - recB - argA。
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