大肠杆菌实验室群体实验进化过程中的染色体变化
Chromosomal changes during experimental evolution in laboratory populations of Escherichia coli.
作者信息
Bergthorsson U, Ochman H
机构信息
Department of Biology, University of Rochester, Rochester, New York 14627, USA.
出版信息
J Bacteriol. 1999 Feb;181(4):1360-3. doi: 10.1128/JB.181.4.1360-1363.1999.
Abstract
Short-term rates of chromosome evolution were analyzed in experimental populations of Escherichia coli B that had been propagated for 2,000 generations under four thermal regimens. Chromosome alterations were monitored in 24 independent populations by pulsed-field gel electrophoresis of DNA treated with five rare-cutting restriction enzymes. A total of 11 changes, 8 affecting chromosome size and 3 altering restriction sites, were observed in these populations, with none occurring in strains cultured at 37 degreesC. Considering the changes detected in these experimental populations, the rate of chromosome alteration of E. coli is estimated to be half of that observed in experimental populations of yeast.
摘要
对在四种热方案下繁殖了2000代的大肠杆菌B实验群体的染色体短期进化速率进行了分析。通过对用五种稀有切割限制酶处理的DNA进行脉冲场凝胶电泳，在24个独立群体中监测染色体变化。在这些群体中总共观察到11处变化，其中8处影响染色体大小，3处改变限制酶切位点，而在37摄氏度培养的菌株中未出现任何变化。考虑到在这些实验群体中检测到的变化，估计大肠杆菌的染色体改变速率是酵母实验群体中观察到的速率的一半。
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