Randolph D A, Carruthers C J, Szabo S J, Murphy K M, Chaplin D D
Center for Immunology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
J Immunol. 1999 Feb 15;162(4):2375-83.
Although evidence is strong that Th cells play a major role in mediating the airway inflammation observed in asthma, the relative contributions of the Th cell subsets, Th1 and Th2, are unclear. It has been suggested that asthma is driven by Th2 predominant responses in the lung, but other data suggest a role for Th1 cells as well. Here we show by intracellular cytokine staining and flow cytometric analysis that in the murine model of OVA-induced airway inflammation, both Th1 and Th2 cells are recruited to the airways. Th1 cells predominate early in the response and Th2 cells predominate late. We further show that increasing the number of Th1 cells by passive transfer of OVA-specific Th1 cells results in increased inflammation. This effect is observed regardless of whether the T cells are transferred before sensitization or after airway inflammation is already in progress. Transfer of Th1 cells also results in increased recruitment of host T cells of both Th1 and Th2 phenotypes. Passive transfer of Th2 cells results in little change in the inflammatory response. These results demonstrate that Ag-specific Th1 cells are not protective in this model of asthma, but rather may potentiate the inflammatory response.
尽管有充分证据表明Th细胞在介导哮喘中观察到的气道炎症方面起主要作用，但Th细胞亚群Th1和Th2的相对贡献尚不清楚。有人提出哮喘是由肺部Th2主导的反应驱动的，但其他数据表明Th1细胞也起作用。在这里，我们通过细胞内细胞因子染色和流式细胞术分析表明，在OVA诱导的气道炎症小鼠模型中，Th1和Th2细胞均被募集到气道。Th1细胞在反应早期占主导，Th2细胞在后期占主导。我们进一步表明，通过被动转移OVA特异性Th1细胞增加Th1细胞数量会导致炎症增加。无论T细胞是在致敏前还是在气道炎症已经发生后转移，都能观察到这种效果。Th1细胞的转移还导致宿主Th1和Th2表型的T细胞募集增加。Th2细胞的被动转移导致炎症反应变化不大。这些结果表明，在该哮喘模型中，抗原特异性Th1细胞没有保护作用，反而可能增强炎症反应。
