一种用于通过DNA模板依赖性聚合酶终止DNA合成的烯丙基/无环腺苷核苷三磷酸。
An allylic/acyclic adenosine nucleoside triphosphate for termination of DNA synthesis by DNA template-dependent polymerases.
作者信息
Martinez C I, Thoresen L H, Gibbs R A, Burgess K
机构信息
Department of Chemistry, PO Box 300012, Texas A & M University, College Station, TX 77842-3012, USA.
出版信息
Nucleic Acids Res. 1999 Mar 1;27(5):1271-4. doi: 10.1093/nar/27.5.1271.
An allylic adenosine triphosphate analog (AATP) was tested as a substrate for commercially available DNA polymerases. All but one of the enzymes assayed incorporated AATP opposite thymidine (T) with concomitant termination of the elongation reaction. A concentration of only 1 microM was sufficient for complete termination of the polymerization reaction for a short template mediated by Ampli Taq DNA polymerase FS (Taq FS). This result suggests that AATP could be used as a 2',3'-dideoxyadenosine-5'-triphosphate (ddA) surrogate. Kinetics of incorporation revealed that AATP was 48 times less efficiently incorporated than ddA. Furthermore, AATP was used in dye-primer sequencing as a substitute for ddA.
一种烯丙基三磷酸腺苷类似物（AATP）被作为市售DNA聚合酶的底物进行测试。在所检测的酶中，除一种酶外，其他所有酶都能在胸腺嘧啶（T）的相对位置掺入AATP，并伴随延伸反应的终止。对于由Ampli Taq DNA聚合酶FS（Taq FS）介导的短模板，仅1微摩尔的浓度就足以使聚合反应完全终止。该结果表明，AATP可作为2'，3'-双脱氧腺苷-5'-三磷酸（ddA）的替代物。掺入动力学表明，AATP的掺入效率比ddA低48倍。此外，AATP被用于染料引物测序中替代ddA。
Zotero 插件