Pai S A, Cheung M C, Romsdahl M M, Multani A S, Pathak S
Department of Cancer Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Cancer Genet Cytogenet. 1999 Feb;109(1):51-7. doi: 10.1016/s0165-4608(98)00152-6.
We evaluated whether genetic instability, which is the hallmark of cancer cells, can be investigated at the single chromosomal level. We established in culture and examined a human malignant melanoma cell line and its 11 distinct clones as well as peripheral blood cultures from the original patient by G-banding, C-banding, and silver-staining (AgNOR) techniques. There were six marker chromosomes common to most of the 11 clones and eight or nine additional marker chromosomes found in only one or in very few clones. Chromosome 1 had a pericentric inversion in the C-banded region in both the tumor and the lymphocyte metaphase spreads. This same homologue was also involved in the formation of one of the shared marker chromosomes; this marker, in turn, was rearranged to form two unique markers in one clone. Our findings suggest that genetic instability can be studied at the single chromosome level. Moreover, this study further supports our earlier contention that peripheral blood lymphocyte cultures can show chromosomal lesions that are stable markers in cancer cells.
我们评估了作为癌细胞标志的基因不稳定性是否能够在单条染色体水平上进行研究。我们建立了培养体系，通过G显带、C显带和银染（AgNOR）技术，对一株人恶性黑色素瘤细胞系及其11个不同的克隆，以及来自原患者的外周血培养物进行了检测。11个克隆中的大多数都有6条标记染色体，另外还有8条或9条标记染色体仅在一个或极少数克隆中出现。在肿瘤细胞和淋巴细胞中期分裂相中，1号染色体在C带区域都发生了臂间倒位。这条同源染色体也参与了一条共享标记染色体的形成；而这条标记染色体又在一个克隆中重排形成了两条独特的标记染色体。我们的研究结果表明，基因不稳定性能够在单条染色体水平上进行研究。此外，这项研究进一步支持了我们之前的观点，即外周血淋巴细胞培养物能够显示出作为癌细胞稳定标记的染色体病变。
