Tan Y H, Tan C, Berthold W
Tex Rep Biol Med. 1977;35:63-8.
Now that some studies have been done on the genetics of the human interferon system, it would appear that the primary constraint to interferon research imposed by the lack of human interferon can be overcome as indicated herein with mutagenized human cell lines containing multiple copies of chromosome 5. Purified interferon from these mutants has allowed us to label it radioactively. However the amount required for a complete amino acid and sugar sequence of human interferon is still very large but within the realm of possibility when the use of these mutant cell lines is considered (23, 24).
既然已经对人类干扰素系统的遗传学进行了一些研究，那么看来，如本文所述，通过含有5号染色体多个拷贝的诱变人类细胞系，可以克服因缺乏人类干扰素而对干扰素研究造成的主要限制。从这些突变体中纯化出的干扰素使我们能够对其进行放射性标记。然而，要获得人类干扰素完整的氨基酸和糖序列所需的量仍然非常大，但考虑到使用这些突变细胞系，这仍是有可能实现的（23, 24）。
