Creagan R P, Tan Y H, Chen S, Ruddle F H
Fed Proc. 1975 Dec;34(13):2222-6.
Current advances in the use of somatic cell hybrid systems have enhanced the value of these systems for studying eukaryotic cell functions. We have reviewed the use of somatic cells to investigate the human interferon system. It has been shown that interspecific heterokaryons and hybrid cells can produce interferon(s) of both parental types and may be protected from viral challenge by interferon(s) from either parent. Using mouse-human hybrid cells we have assigned a human gene(s) responsible for regulating interferon to chromosome 21 and genes involved in the production of human interferon to chromosomes 2 and 5. Our data also suggest possible assignment of a locus involved in control of interferon production to chromosome 16. Suggested further uses of the somatic cell system for interferon studies include study of the subunit structure of interferons and the development of hybrid lines that produce human interferon at high levels (interferon/somatic cell hybrids/human gene assignment.
体细胞杂交系统应用方面的当前进展提升了这些系统在研究真核细胞功能中的价值。我们回顾了利用体细胞来研究人类干扰素系统的情况。已表明种间异核体和杂交细胞可产生双亲类型的干扰素，并且可能受到任一亲本产生的干扰素的保护而免受病毒攻击。利用小鼠 - 人杂交细胞，我们已将负责调节干扰素的人类基因定位到21号染色体，将参与人类干扰素产生的基因定位到2号和5号染色体。我们的数据还表明，可能将参与干扰素产生控制的一个基因座定位到16号染色体。体细胞系统在干扰素研究中的进一步应用建议包括干扰素亚基结构的研究以及开发能高水平产生人类干扰素的杂交细胞系（干扰素/体细胞杂交/人类基因定位）。
