Tavassoli M, Bening J, Houchin D, Crosby W H
Am J Pathol. 1976 Nov;85(2):363-72.
An experimental model is presented whereby erythroblastemia, the appearance of nucleated red cells in circulation, was induced reproducibly in rabbits by intravenous injection of 0.5 to 1 g/kg carbonyl iron particles (CIP). The phenomenon reached a peak in 24 to 48 hours and disappeared after a week. Erythroblastemia was associated with evidence for disseminated intravenous coagulation; both could be aborted by administration of heparin. Repeated injection of CIP was always followed by recurrence of erythroblastemia. For a period as long as 7 months, the animals tolerated repeated injections without evidence of lasting sequelae. Electron microscopic studies of marrow tissue indicated alterations in the red cell nuclei but no alterations in the endothelium of marrow sinuses into which the red cells migrate to reach the circulation. This model provides a means to study the mechanisms involved in nuclear elimination by mammalian red cells.
本文提出了一种实验模型，通过静脉注射0.5至1 g/kg羰基铁颗粒（CIP），可在兔体内反复诱导出循环中出现有核红细胞的成红细胞血症。该现象在24至48小时达到峰值，并在一周后消失。成红细胞血症与弥散性静脉内凝血的证据相关；两者均可通过给予肝素而中止。重复注射CIP总是会导致成红细胞血症复发。在长达7个月的时间里，动物耐受重复注射，未出现持久后遗症的迹象。对骨髓组织的电子显微镜研究表明红细胞核有改变，但红细胞迁移至循环所经过的骨髓窦内皮没有改变。该模型提供了一种研究哺乳动物红细胞核消除机制的方法。
