Aarestrup F M, Carstensen B
Danish Veterinary Laboratory, Copenhagen V.
Microb Drug Resist. 1998 Winter;4(4):307-12. doi: 10.1089/mdr.1998.4.307.
This study was conducted to investigate the effect of tylosin used as a growth promoter on the occurrence of macrolide-resistant enterococci and staphylococci in pigs. Two identical feeding experiments with 10 pigs each were conducted to evaluate the effect of tylosin on the occurrence of erythromycin resistance among enterococci sampled from feces and Staphylococcus hyicus sampled by skin swabs. Half of the pigs were fed antibiotic-free feed and the other half feed with tylosin (30 microg/g) added. For each pig, weekly fecal samples and skin swabs were collected and examined for erythromycin-susceptible and erythromycin-resistant enterococci and S. hyicus, respectively. There was an immediate effect of tylosin on the fraction of resistant enterococci recovered, increasing by a factor 2.4; the effect on S. hyicus was more gradual, increasing at a rate of about 8% per day and totaling a five-fold increase over 20 days. Thus, a clear effect on resistance occurrence was demonstrated, not only in intestinal bacteria, but also on bacteria sampled from the skin.
本研究旨在调查作为生长促进剂使用的泰乐菌素对猪体内耐大环内酯类肠球菌和葡萄球菌发生情况的影响。进行了两项相同的饲养实验，每项实验有10头猪，以评估泰乐菌素对从粪便中采集的肠球菌以及通过皮肤拭子采集的猪葡萄球菌中红霉素耐药性发生情况的影响。一半的猪饲喂不含抗生素的饲料，另一半饲喂添加了泰乐菌素（30微克/克）的饲料。对于每头猪，每周采集粪便样本和皮肤拭子，分别检测对红霉素敏感和耐药的肠球菌以及猪葡萄球菌。泰乐菌素对回收的耐药肠球菌比例有立竿见影的效果，增加了2.4倍；对猪葡萄球菌的影响较为缓慢，每天以约8%的速度增加，20天内总计增加了五倍。因此，不仅在肠道细菌中，而且在从皮肤采集的细菌中，都证明了对耐药性发生有明显影响。
