核糖体失活蛋白：皂草素的纯化、结晶及初步X射线衍射研究

Purification, crystallisation and preliminary X-ray diffraction study of ribosome inactivating protein: saporin.

作者信息

Kumar M, Dattagupta S, Kannan K K, Hosur M V

机构信息

Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.

出版信息

Biochim Biophys Acta. 1999 Jan 11;1429(2):506-11. doi: 10.1016/s0167-4838(98)00267-2.

DOI:10.1016/s0167-4838(98)00267-2

PMID:9989237

Abstract

We report here the crysallisation and molecular replacement results on the structure determination of S-9 isoform of the ribosome inactivating protein saporin. The protein was purified to homogeneity by a simple and efficient protocol. The crystals belong to the space group I4l with a = b = 91.47 A, c = 150.66 A and contain two molecules in the asymmetric unit.

摘要

我们在此报告核糖体失活蛋白皂草素S-9亚型结构测定的结晶和分子置换结果。该蛋白通过一种简单有效的方案纯化至同质。晶体属于空间群I41，a = b = 91.47 Å，c = 150.66 Å，不对称单元中含有两个分子。