肥皂草中核糖体失活蛋白皂草素的结晶及X射线初步研究

Crystallization and preliminary X-ray study of saporin, a ribosome-inactivating protein from Saponaria officinalis.

作者信息

Savino C, Federici L, Brancaccio A, Ippoliti R, Lendaro E, Tsernoglou D

机构信息

Department of Biochemical Sciences and CNR, Centre for Molecular Biology, Università di Roma 'La Sapienza', Piazzale Aldo Moro 5, 00185 Rome, Italy.

出版信息

Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):636-8. doi: 10.1107/s0907444997012249.

DOI:10.1107/s0907444997012249

PMID:9761860

Abstract

Single crystals of the protein saporin isolated from the seeds of S. officinalis have been grown by the vapor-diffusion method using ammonium sulfate as precipitant. The crystals are tetragonal, space group P4122 (P4322), with cell dimensions a = b = 67.53 and c = 119. 67 A, and diffract to 2.0 A resolution on a rotating-anode X-ray source. The asymmetric unit contains one molecule, corresponding to a volume of the asymmetric unit per unit mass (Vm) of 2.38 A3 Da-1.

摘要

已通过气相扩散法，以硫酸铵作为沉淀剂，培养出了从药用肥皂草种子中分离得到的蛋白质皂草素的单晶。晶体为四方晶系，空间群为P4122（P4322），晶胞参数a = b = 67.53 Å，c = 119.67 Å，在旋转阳极X射线源上衍射至2.0 Å分辨率。不对称单元包含一个分子，对应于每单位质量不对称单元的体积（Vm）为2.38 Å3 Da-1。