Savino C, Federici L, Brancaccio A, Ippoliti R, Lendaro E, Tsernoglou D
Department of Biochemical Sciences and CNR, Centre for Molecular Biology, Università di Roma 'La Sapienza', Piazzale Aldo Moro 5, 00185 Rome, Italy.
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):636-8. doi: 10.1107/s0907444997012249.
Single crystals of the protein saporin isolated from the seeds of S. officinalis have been grown by the vapor-diffusion method using ammonium sulfate as precipitant. The crystals are tetragonal, space group P4122 (P4322), with cell dimensions a = b = 67.53 and c = 119. 67 A, and diffract to 2.0 A resolution on a rotating-anode X-ray source. The asymmetric unit contains one molecule, corresponding to a volume of the asymmetric unit per unit mass (Vm) of 2.38 A3 Da-1.
已通过气相扩散法，以硫酸铵作为沉淀剂，培养出了从药用肥皂草种子中分离得到的蛋白质皂草素的单晶。晶体为四方晶系，空间群为P4122（P4322），晶胞参数a = b = 67.53 Å，c = 119.67 Å，在旋转阳极X射线源上衍射至2.0 Å分辨率。不对称单元包含一个分子，对应于每单位质量不对称单元的体积（Vm）为2.38 Å3 Da-1。
