Moya-Camarena S Y, Van den Heuvel J P, Belury M A
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA.
Biochim Biophys Acta. 1999 Jan 4;1436(3):331-42. doi: 10.1016/s0005-2760(98)00121-0.
Since conjugated linoleic acid (CLA) has structural and physiological characteristics similar to peroxisome proliferators, we hypothesized that CLA would activate peroxisome proliferator-activated receptor (PPAR). We compared the effects of dietary CLA (0.0, 0.5, 1.0 and 1.5% by weight) with a peroxisome proliferator (0.01% Wy-14,643) in female and male Sprague-Dawley (SD) rats. Dietary CLA had little effect on body weight, liver weight, and hepatic peroxisome proliferation, compared to male rats fed Wy-14,643 diet. Lipid content in livers from rats fed 1.5% CLA and Wy-14,643 diets was increased (P < 0.01) when compared to rats fed control diets regardless of gender. Hepatic acyl-CoA oxidase (ACO) mRNA levels were increased 3-fold in male rats fed 1.5% CLA diet compared to rats fed control diets while Wy-14,643 supported approximately 30-fold ACO mRNA accumulation. A similar response was observed for liver fatty acid-binding protein (L-FABP) mRNA. The effect of dietary treatments on hepatic PPAR-responsive genes in female rats was weaker than in male rats. The (9Z,11E)-CLA isomer activated PPAR alpha in transfected cells to a similar extent as Wy-14,643, whereas the furan-CLA metabolite was comparable to bezafibrate on activating PPAR beta. These data suggest that while CLA was able to activate PPARs it is not a peroxisome proliferator in SD rats.
由于共轭亚油酸（CLA）具有与过氧化物酶体增殖剂相似的结构和生理特征，我们推测CLA会激活过氧化物酶体增殖物激活受体（PPAR）。我们比较了膳食CLA（按重量计0.0%、0.5%、1.0%和1.5%）与过氧化物酶体增殖剂（0.01% Wy-14,643）对雌性和雄性斯普拉格-道利（SD）大鼠的影响。与喂食Wy-14,643饮食的雄性大鼠相比，膳食CLA对体重、肝脏重量和肝脏过氧化物酶体增殖几乎没有影响。无论性别，与喂食对照饮食的大鼠相比，喂食1.5% CLA和Wy-14,643饮食的大鼠肝脏中的脂质含量增加（P < 0.01）。与喂食对照饮食的大鼠相比，喂食1.5% CLA饮食的雄性大鼠肝脏酰基辅酶A氧化酶（ACO）mRNA水平增加了3倍，而Wy-14,643支持ACO mRNA积累约30倍。肝脏脂肪酸结合蛋白（L-FABP）mRNA也观察到类似的反应。饮食处理对雌性大鼠肝脏PPAR反应性基因的影响比雄性大鼠弱。（9Z,11E）-CLA异构体在转染细胞中激活PPARα的程度与Wy-14,643相似，而呋喃-CLA代谢物在激活PPARβ方面与苯扎贝特相当。这些数据表明，虽然CLA能够激活PPAR，但它在SD大鼠中不是过氧化物酶体增殖剂。
