Juránek I, Suzuki H, Yamamoto S
Department of Biochemistry, Tokushima University School of Medicine, Japan.
Biochim Biophys Acta. 1999 Jan 4;1436(3):509-18. doi: 10.1016/s0005-2760(98)00159-3.
In an attempt to study affinities for molecular oxygen of mammalian arachidonate oxygenases, which remain unclarified at present, we determined activities of platelet-type 12-lipoxygenase, leukocyte-type 12-lipoxygenase, 5-lipoxygenase, 15-lipoxygenase, cyclooxygenase-1 and cyclooxygenase-2 at various oxygen concentrations. Activities of all the tested enzymes were assessed by oxygenation of radioactive arachidonic acid under hypoxic conditions, and part of the enzymes were also assayed by monitoring oxygen consumption. Their Km values for oxygen ranged between 10 and 26 microM. These results should be considered in investigations of arachidonic acid metabolism in pathophysiological processes associated with hypoxia.
为了研究目前仍未明确的哺乳动物花生四烯酸加氧酶对分子氧的亲和力，我们测定了血小板型12-脂氧合酶、白细胞型12-脂氧合酶、5-脂氧合酶、15-脂氧合酶、环氧化酶-1和环氧化酶-2在不同氧浓度下的活性。所有受试酶的活性通过在缺氧条件下对放射性花生四烯酸进行氧合来评估，部分酶还通过监测耗氧量进行测定。它们对氧的米氏常数在10至26微摩尔之间。在与缺氧相关的病理生理过程中研究花生四烯酸代谢时应考虑这些结果。
