Fujino A, Hori H, Higashi T, Morimoto Y, Tanaka I, Kaji H
Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Kitakyushu 807, Japan.
Int J Occup Environ Health. 1995 Jan;1(1):21-28. doi: 10.1179/oeh.1995.1.1.21.
The potential toxicities of fibrous materials were investigated by measuring the levels of cytokines as well as cytoplasmic and lysosomal enzymes released from alveolar macrophages (AMs) in vitro. Five man-made mineral fibers (ceramic, glass, potassium octatitanate, and two magnesium sulfate whiskers), as well as five natural mineral fibers (UICC chrysotile, crocidolite, amosite, anthophylite, and Turkish erionite) were tested. Basic fiber characteristics, including fiber size, surface area, number, and solubility, were also measured. Tumor necrosis factor (TNF) production, lactate dehydrogenase (LDH) and beta-glucuronidase (BGU) release from AMs exposed to potassium octatitanate, magnesium sulfate whiskers, and ceramic fiber correlated with pathologic changes in the lung according to inhalation studies. The solubility of the man-made mineral fibers also correlated with the half-life of clearance in an in-vivo study. The results suggest that measured values of TNF production, LDH and BGU release in vitro, and fiber solubility combined are a good indicator of the pathogenic potential of fibers in vivo.
通过在体外测量细胞因子水平以及肺泡巨噬细胞（AM）释放的细胞质和溶酶体酶，研究了纤维材料的潜在毒性。测试了五种人造矿物纤维（陶瓷、玻璃、八钛酸钾以及两种硫酸镁晶须）以及五种天然矿物纤维（国际癌症研究机构（UICC）温石棉、青石棉、铁石棉、直闪石和土耳其毛沸石）。还测量了包括纤维尺寸、表面积、数量和溶解度在内的基本纤维特性。根据吸入研究，暴露于八钛酸钾、硫酸镁晶须和陶瓷纤维的AM产生的肿瘤坏死因子（TNF）、释放的乳酸脱氢酶（LDH）和β-葡萄糖醛酸酶（BGU）与肺部的病理变化相关。在一项体内研究中，人造矿物纤维的溶解度也与清除半衰期相关。结果表明，体外测量的TNF产生、LDH和BGU释放值以及纤维溶解度相结合，是纤维在体内致病潜力的良好指标。
