Schichnes D, Nemson J, Sohlberg L, Ruzin SE
CNR Biological Imaging Facility, Department of Plant and Microbial Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, CA 94720
Microsc Microanal. 1998 Sep;4(5):491-496. doi: 10.1017/s1431927698980461.
: We have developed a microwave protocol for a paraffin-embedding microtechnique of the shoot apical meristem of Zea mays and have successfully applied this protocol to other plant tissues. This protocol decreases the time required for all aspects of microtechnique tissue processing, including fixation (24 hr to 15 min), dehydration (73 hr to 10 min), and infiltration (96 hr to 3 hr). Additionally, the time required to adhere paraffin ribbons to gelatin-coated slides and for the Johanson's safranin O, fast green FCF staining protocol has been significantly decreased. Using this technique, the quality of tissue preservation and subsequent in situ localization of knotted mRNA was increased by using microwaves.
我们已经开发出一种用于玉米茎尖分生组织石蜡包埋微技术的微波方案，并已成功将该方案应用于其他植物组织。该方案减少了微技术组织处理各个方面所需的时间，包括固定（从24小时减少到15分钟）、脱水（从73小时减少到10分钟）和渗透（从96小时减少到3小时）。此外，将石蜡切片粘贴到涂有明胶的载玻片上以及进行约翰逊氏番红O、固绿FCF染色方案所需的时间也显著减少。使用该技术，通过微波提高了组织保存的质量以及随后打结mRNA的原位定位效果。
