石油降解微生物的计数
Enumeration of petroleum-degrading microorganisms.
作者信息
Walker J D, Colwell R R
出版信息
Appl Environ Microbiol. 1976 Feb;31(2):198-207. doi: 10.1128/aem.31.2.198-207.1976.
Abstract
A variety of factors, including concentration of oil, antibiotics, dyes, and inoculum washes, were examined to determine their effect on the total counts of microorganisms on oil-containing media. The media found to be best for enumerating petroleum-degrading microorganisms contained 0.5% (vol/vol) oil and 0.003% phenol red, with Fungizone added for isolating bacteria and streptomycin and tetracycline added for isolating yeasts and fungi. Washing the inoculum did not improve recovery of petroleum degraders. Specifically, silica gel-oil medium and a yeast medium are recommended for enumeration of petroleum-degrading bacteria and yeasts and fungi, respectively. It is suggested that counts of petroleum degraders be expressed as percentage of the total population rather than total numbers of petroleum degraders per se. Incubation temperature and presence of oil was found to influence the numbers of petroleum-degrading microorganisms at a given sampling site.
摘要
研究了多种因素，包括油的浓度、抗生素、染料和接种物洗涤，以确定它们对含油培养基上微生物总数的影响。发现最适合计数石油降解微生物的培养基含有0.5%（体积/体积）的油和0.003%的酚红，添加两性霉素用于分离细菌，添加链霉素和四环素用于分离酵母和真菌。洗涤接种物并不能提高石油降解菌的回收率。具体而言，建议分别使用硅胶 - 油培养基和酵母培养基来计数石油降解细菌以及酵母和真菌。建议将石油降解菌的计数表示为总菌数的百分比，而不是石油降解菌本身的总数。发现在给定采样点，培养温度和油的存在会影响石油降解微生物的数量。
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