Carney J F, Colwell R R
Appl Environ Microbiol. 1976 Feb;31(2):227-33. doi: 10.1128/aem.31.2.227-233.1976.
Uptake of 14C-labeled glucose and glutamate was studied at several sites in Baltimore Harbor, Eastern Bay, and the Rhode River. Levels of uptake of 14C-labeled glutamate, measured over a period of 1 year during September, December, April, May, and June to estimate seasonal effects on heterotrophic utilization of selected nutrients, were highest in May and lowest in December. In a comparison of visibly polluted and unpolluted sites, the greatest amount of incorporation of glucose or glutamate and the highest Vmax values were observed at those sites where the microorganisms were exposed to varied and higher levels of pollutants, suggesting that Vmax may function as an indicator of relative pollution. Mineralization values ranged from 30 to 43%. A range of 0.44 to 2.32 mumol of C/liter per day was calculated for glutamate uptake.
在巴尔的摩港、东湾和罗德河的几个地点研究了14C标记的葡萄糖和谷氨酸的摄取情况。为了评估季节对选定营养物质异养利用的影响，在9月、12月、4月、5月和6月期间的1年时间内测量了14C标记的谷氨酸摄取水平，5月最高，12月最低。在对明显污染和未污染地点的比较中，在微生物接触到不同且更高水平污染物的那些地点，观察到葡萄糖或谷氨酸的最大掺入量和最高Vmax值，这表明Vmax可能作为相对污染的指标。矿化值范围为30%至43%。计算出谷氨酸摄取量为每天0.44至2.32微摩尔碳/升。
