测量烃降解细菌的潜在活性。
Measuring the potential activity of hydrocarbon-degrading bacteria.
作者信息
Walker J D, Colwell R R
出版信息
Appl Environ Microbiol. 1976 Feb;31(2):189-97. doi: 10.1128/aem.31.2.189-197.1976.
Abstract
[14C]hydrocarbons were utilized as a means of estimating the hydrocarbon-degrading potential of bacteria in estuarine and marine environments. Evaporation of the hydrocarbons must be considered in estimates of oxidation. Amount of mineralization of [14C]hexadecane can be equated with the total number of petroleum-degrading bacteria and the percentage of the total heterotrophic population, which they represent. Mineralization activity was found to be related to the activity of the bacterial populations during in situ incubation. Rates of mineralization were observed, as follows, for [14C]hexadecane greater than [14C]naphthalene greater than [14C]toluene greater than [14C]cyclohexane. Increased rates of uptake and mineralization were observed for bacteria in samples collected from an oil-polluted harbor compared with samples from a relatively unpolluted, shellfish-harvesting area, e.g., turnover times of 15 and 60 min for these areas, respectively, using [14C]hexadecane.
摘要
[14C]碳氢化合物被用作评估河口和海洋环境中细菌降解碳氢化合物潜力的一种手段。在氧化估计中必须考虑碳氢化合物的蒸发。[14C]十六烷的矿化量可以等同于石油降解细菌的总数以及它们所代表的总异养菌种群的百分比。发现矿化活性与原位培养期间细菌种群的活性相关。观察到[14C]十六烷、[14C]萘、[14C]甲苯、[14C]环己烷的矿化速率如下：[14C]十六烷＞[14C]萘＞[14C]甲苯＞[14C]环己烷。与来自相对未受污染的贝类捕捞区的样品相比，从受油污染港口采集的样品中的细菌摄取和矿化速率有所增加，例如，使用[14C]十六烷时，这些区域的周转时间分别为15分钟和60分钟。
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