Doughty M J, Dodd G H
Biochim Biophys Acta. 1976 Dec 21;451(2):592-603. doi: 10.1016/0304-4165(76)90154-9.
(1) Evidence is presented which indicates that the carbocyanine dye (3,3'-dipropyl thiadicarbocyanine) can be used as a spectroscopic probe for monitoring the resting potential across tha plasma membrane of the ciliated protozoan Paramecium. (2) The dye at low concentrations (less than or equal to 1 muM) does not affect either the viability or the motility of the cells, nor does it induce a chemotactic response. (3) The fluorescence of the dye bound to the cells alters as the potential across the membrane is changed by increasing the external cation concentration. (4) The absorbance of the bound dye also changes in response to an alteration of the membrane potential. (5) The membrane potential changes as measured by the fluorescence method have been correlated with the measurements of the potential estimated by microelectrode methods. (6) Both cations which induce a negative chemotactic response in Paramecium (K+, Na+, Ba2+) and several non-toxic cations bring about a rapid depolarization of the plasma membrane. The significance of these rapid changes in relation to the swimming behaviour of the ciliate is discussed.
（1）有证据表明，羰花青染料（3,3'-二丙基硫代二羰花青）可用作光谱探针，以监测纤毛原生动物草履虫质膜上的静息电位。（2）低浓度（小于或等于1μM）的该染料既不影响细胞的活力或运动性，也不诱导趋化反应。（3）随着膜电位因外部阳离子浓度增加而改变，与细胞结合的染料的荧光也会改变。（4）结合染料的吸光度也会随着膜电位的改变而变化。（5）通过荧光法测量的膜电位变化已与通过微电极法估计的电位测量结果相关联。（6）在草履虫中诱导负趋化反应的阳离子（K +、Na +、Ba2 +）以及几种无毒阳离子都会导致质膜迅速去极化。讨论了这些快速变化与纤毛虫游泳行为的关系。
