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脂质双分子层膜的电压诱导电容弛豫。膜组成的影响。

Voltage-induce capacitance relaxation of lipid bilayer membranes. Effects of membrane composition.

作者信息

Benz R, Janko K

出版信息

Biochim Biophys Acta. 1976 Dec 14;455(3):721-38. doi: 10.1016/0005-2736(76)90043-2.

DOI:10.1016/0005-2736(76)90043-2
PMID:999936
Abstract

The specific capacity of black lipid membranes of different phospholipids dissolved in n-alkanes was investigated. The hydrocarbon thickness of these membranes, as calculated from the electric capacity with a dielectric constant of 2.1, was in most cases close to 5 nm. It was found that the specific capacity is not constant with time after blackening. It shows a linear time dependence characteristic for each lipid/solvent system. The influence of a transmembrane potential on the capacity of the membranes was measured. It was shown that the extent of the capacity change, obtained 10 s after applying a voltage, was strongly dependent on the lipid composition as well as the solvent content of the membranes. The capacity change of the membranes seems to be caused mainly by a thickness change and not by an area increase of the membranes.

摘要

研究了溶解于正构烷烃中的不同磷脂的黑色脂质膜的比电容。根据介电常数为2.1时的电容计算得出,这些膜的烃厚度在大多数情况下接近5nm。发现黑化后比电容并非随时间恒定不变。它对每个脂质/溶剂体系呈现出线性时间依赖性特征。测量了跨膜电位对膜电容的影响。结果表明,施加电压10s后获得的电容变化程度强烈依赖于脂质组成以及膜的溶剂含量。膜的电容变化似乎主要是由厚度变化而非膜面积增加引起的。

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