DeSombre E R, Lyttle C R
Cancer Res. 1978 Nov;38(11 Pt 2):4086-90.
7,12-Dimethylbenz(a)anthracene-induced rat mammary tumors often contain high levels of the enzyme perioxidase, a putative marker of estrogen dependence. This enzyme can be effectively extracted with 0.5 M CaCl2, giving rise to a soluble peroxidase with a molecular weight of about 50,000 as determined by gel filtration. This is the same size as the estrogen-induced peroxidase of rat uterus but smaller than other mammalian peroxidases. Further purification of the rat mammary tumor peroxidase by concanavalin A-Sepharose chromatography and hydrophobic interaction chromatography on phenyl Sepharose provides a 640-fold purification of the enzyme.
7,12-二甲基苯并(a)蒽诱导的大鼠乳腺肿瘤通常含有高水平的过氧化物酶，这是一种雌激素依赖性的假定标志物。这种酶可用0.5M氯化钙有效提取，通过凝胶过滤测定，产生一种分子量约为50,000的可溶性过氧化物酶。这与大鼠子宫中雌激素诱导的过氧化物酶大小相同，但比其他哺乳动物的过氧化物酶小。通过伴刀豆球蛋白A-琼脂糖层析和苯基琼脂糖上的疏水相互作用层析对大鼠乳腺肿瘤过氧化物酶进行进一步纯化，可使该酶纯化640倍。
