Kim F Y, Goldstein M
Department of Urology, New York Hospital-Cornell Medical Center, New York 10021, USA.
J Urol. 1999 Mar;161(3):819-21.
Antibacterial skin preparation was used before obtaining semen cultures to assess whether true seminal pathogens could be isolated with greater specificity.
A total of 14 paired semen cultures were obtained from men evaluated for infertility. The first set (group 1) was obtained without preparation and the second set (group 2) was obtained after using an antibacterial skin preparation consisting of a shower followed by perineal, penile and hand wash with 4% chlorhexidine and 10% povidone-iodine. The sets were compared and statistical analysis was performed using the paired t test.
In group 1, 25 total bacterial isolates were detected compared to 13 in group 2, which was statistically significant (p <0.002). In group 1, 11 enteric organisms were isolated compared to 5 in group 2, which was also statistically significant (p <0.05). These data suggest that the source of most enteric organisms isolated from semen is genital and perineal skin.
A significant reduction in enteric contaminants, which would otherwise be erroneously treated, can be achieved with an effective antibacterial skin preparation. We were able to isolate true pathogens with greater specificity with (80%) than without (63%) an antibacterial skin preparation. An antibacterial skin preparation reduces false-positive cultures by at least 50%, which would lead to a significant reduction in costs and morbidity.
在获取精液培养物之前使用抗菌皮肤准备措施，以评估是否能更具特异性地分离出真正的精液病原体。
从接受不育评估的男性中总共获取了14对精液培养物。第一组（第1组）在未进行准备的情况下获取，第二组（第2组）在使用抗菌皮肤准备措施后获取，该措施包括淋浴，然后用4%洗必泰和10%聚维酮碘对会阴部、阴茎和手部进行清洗。对两组进行比较，并使用配对t检验进行统计分析。
第1组检测到总共25株细菌分离株，而第2组为13株，差异具有统计学意义（p<0.002）。第1组分离出11株肠道微生物，第2组为5株，同样具有统计学意义（p<0.05）。这些数据表明，从精液中分离出的大多数肠道微生物来源是生殖器和会阴部皮肤。
通过有效的抗菌皮肤准备措施，可以显著减少否则会被错误治疗的肠道污染物。我们使用抗菌皮肤准备措施（80%）比不使用（63%）能够更具特异性地分离出真正的病原体。抗菌皮肤准备措施可将假阳性培养结果至少降低50%，这将显著降低成本和发病率。
