Damirayakhian M, Jeyendran R S, Land S A
Andrology Laboratory Services Inc., Chicago, Illinois 60611, USA.
Arch Androl. 2006 Jul-Aug;52(4):239-42. doi: 10.1080/01485010600630132.
Semen cultures often yield inconclusive results probably due to bacterial contaminants. We were able to isolate enteric pathogens with greater specificity by comparing two cultures from the same individual: one obtained from the midstream urine and the other from ejaculate obtained following midstream urine collection and eliminating the bacteria present in the midstream urine. Bacterial growth was found in semen cultures from 102 (79%) of the 129 men with questionable semen quality; 31 (30%) had aerobic bacteria, 28 (27%) had anaerobic bacteria, and 43 (42%) had both. A high incidence of positive cultures in our study was probably due to stringent patient selection and our comparative culturing procedure. The presence of pathogens may indicate the need for treatment especially prior to IVF and IUI.
精液培养结果往往不确定，这可能是由于细菌污染所致。通过比较同一个体的两种培养物，我们能够更具特异性地分离出肠道病原体：一种是取自中段尿，另一种是在收集中段尿后获取的射精样本，并去除中段尿中存在的细菌。在129名精液质量存疑的男性中，102名（79%）的精液培养发现有细菌生长；31名（30%）有需氧菌，28名（27%）有厌氧菌，43名（42%）两者皆有。我们研究中培养阳性的高发生率可能归因于严格的患者选择和我们的比较培养程序。病原体的存在可能表明需要进行治疗，尤其是在体外受精和宫腔内人工授精之前。
