Mitchell P J, Pitt Ford T R, Torabinejad M, McDonald F
Department of Conservative Dentistry, GKT School of Dentistry, Guy's Hospital, London, UK.
Biomaterials. 1999 Jan;20(2):167-73. doi: 10.1016/s0142-9612(98)00157-4.
This study investigated the biocompatibility of variants of mineral trioxide aggregate (MTA), by culturing human MG63 osteosarcoma cells in the presence of materials, observing cytomorphology and cell growth, and then assaying cytokine expression from the cells. Reference materials were employed. Cell growth was quantified by preparing samples (n = 6) at 2, 4 and 7 days, for viewing by scanning electron microscopy and then scoring the amount of material that was covered by healthy cells. Subsequently, samples of culture media were tested using ELISA assays for expression of Interleukin (IL)-1alpha, IL-6, IL-8, IL-11 and macrophage colony stimulating factor (M-CSF). These assays were compared with controls where no material was present, and where media and fetal calf serum had not been exposed to cells. Results showed good cell growth on MTA. Expression of IL-6 from cells was only evident in the presence of MTA and Interpore 200. Interleukin-8 was expressed in high concentrations only in the presence of MTA. There was no evidence of expression of IL-1alpha or IL-11 with any material. Production of M-CSF was high for all materials. It appears that the variants of MTA are biocompatible and suitable for use in clinical trials.
本研究通过在材料存在的情况下培养人MG63骨肉瘤细胞、观察细胞形态和细胞生长，然后检测细胞中的细胞因子表达，来研究三氧化矿物凝聚体（MTA）变体的生物相容性。使用了参考材料。通过在第2天、第4天和第7天制备样本（n = 6）来量化细胞生长，用于扫描电子显微镜观察，然后对被健康细胞覆盖的材料量进行评分。随后，使用酶联免疫吸附测定（ELISA）检测培养基样本中白细胞介素（IL）-1α、IL-6、IL-8、IL-11和巨噬细胞集落刺激因子（M-CSF）的表达。将这些测定结果与不存在材料以及培养基和胎牛血清未接触细胞的对照进行比较。结果显示MTA上细胞生长良好。细胞中IL-6的表达仅在MTA和Interpore 200存在时明显。白细胞介素-8仅在MTA存在时高浓度表达。没有证据表明任何材料存在时IL-1α或IL-11的表达。所有材料的M-CSF产生量都很高。看来MTA变体具有生物相容性，适合用于临床试验。
