一种牙本质替代骨水泥对人MG63成骨细胞的体外生物相容性：BioDentine™与MTA(®)的比较

In vitro biocompatibility of a dentine substitute cement on human MG63 osteoblasts cells: Biodentine™ versus MTA(®).

作者信息

Attik G N, Villat C, Hallay F, Pradelle-Plasse N, Bonnet H, Moreau K, Colon P, Grosgogeat B

机构信息

Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1, Villeurbanne, France.

出版信息

Int Endod J. 2014 Dec;47(12):1133-41. doi: 10.1111/iej.12261. Epub 2014 Mar 20.

DOI:10.1111/iej.12261

PMID:24517569

Abstract

AIM

To compare the in vitro biocompatibility of Biodentine™ and White ProRoot(®) mineral trioxide aggregate (MTA(®) ) with MG63 osteoblast-like cells and to characterize the cement surface.

METHODOLOGY

A direct contact model for MG63 osteoblast-like cells with cements was used for 1, 3 and 5 days. Four end-points were investigated: (i) cement surface characterization by atomic force microscopy (AFM), (ii) cell viability by MTT assay, (iii) protein amount quantification by Bradford assay and (iv) cell morphology by SEM. Statistical analyses were performed by analysis of variance (anova) with a repetition test method.

RESULTS

The roughness of the cements was comparable as revealed by AFM analysis. The MTT test for Biodentine™ was similar to that of MTA(®) . Biodentine™ and MTA(®) induced a similar but slight decrease in metabolic activity. The amount of total protein was significantly enhanced at day three (P < 0.05) but slightly decreased at day five for both tested samples. Biodentine™ was tolerated as well as MTA(®) in all cytotoxicity assays. SEM observations showed improvement of cell attachment and proliferation on both material surfaces following the three incubation periods.

CONCLUSION

The biocompatibility of Biodentine™ to bone cells was comparable to MTA(®) .

摘要

目的

比较Biodentine™和白色ProRoot（®）三氧化矿物凝聚体（MTA（®））与MG63成骨样细胞的体外生物相容性，并对两种骨水泥表面进行表征。

方法

采用MG63成骨样细胞与骨水泥的直接接触模型，培养1、3和5天。研究了四个终点指标：（i）通过原子力显微镜（AFM）对骨水泥表面进行表征；（ii）通过MTT法检测细胞活力；（iii）通过Bradford法对蛋白量进行定量；（iv）通过扫描电子显微镜（SEM）观察细胞形态。采用方差分析（anova）和重复检验方法进行统计学分析。

结果

AFM分析显示，两种骨水泥的粗糙度相当。Biodentine™的MTT试验结果与MTA（®）相似。Biodentine™和MTA（®）均导致代谢活性出现相似但轻微的下降。在第三天，两个测试样本的总蛋白量均显著增加（P < 0.05），而在第五天则略有下降。在所有细胞毒性试验中，Biodentine™与MTA（®）一样具有良好的耐受性。SEM观察表明，经过三个培养期后，两种材料表面的细胞附着和增殖情况均有所改善。