Division of Microbiology, Wuhan Center for Disease Control and Prevention, Wuhan 430024, China.
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2023 Jul 29;24(15):12189. doi: 10.3390/ijms241512189.
Group A rotaviruses (RVAs) are major etiologic agents of gastroenteritis in infants and young children worldwide. To study the prevalence and genetic characteristics of RVAs, a hospital-based surveillance study was conducted in Wuhan, China from June 2019 through May 2022. The detection rates of RVAs were 19.40% (142/732) and 3.51% (8/228) in children and adults, respectively. G9P[8] was the predominant genotype, followed by G8P[8] and G3P[8]. G8P[8] emerged and was dominant in the 2021-2022 epidemic season. The genome constellation of six G8P[8] strains was assigned to G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that the VP7, VP4, VP2, VP3, NSP1, NSP2, NSP3, and NSP5 genes of these G8P[8] strains clustered closely with those of the G8P[8] strains in Asia and were distant from those of the P[8] and G2P[4] strains simultaneously detected in Wuhan. In contrast, the VP1, VP6, and NSP4 genes were closely related to the typical G2P[4] rotavirus, including those of G2P[4] strains simultaneously detected in Wuhan. The detection rate of RVAs decreased in the COVID-19 pandemic era. It was deduced that the G8P[8] rotaviruses that emerged in China may be reassortants, carrying the VP6, VP1, and NSP4 genes derived from the G2P[4] rotavirus in the backbone of the neighboring DS-1-like G8P[8] strains represented by CAU17L-103.
A 组轮状病毒（RVAs）是全球婴幼儿胃肠炎的主要病原体。为了研究 RVAs 的流行率和遗传特征，我们在中国武汉进行了一项基于医院的监测研究，时间为 2019 年 6 月至 2022 年 5 月。在儿童和成人中，RVA 的检出率分别为 19.40%（142/732）和 3.51%（8/228）。G9P[8]是主要的基因型，其次是 G8P[8]和 G3P[8]。G8P[8]在 2021-2022 流行季出现并占主导地位。六株 G8P[8]的基因组构成被分配到 G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2。系统进化分析显示，这些 G8P[8]株的 VP7、VP4、VP2、VP3、NSP1、NSP2、NSP3 和 NSP5 基因与亚洲 G8P[8]株的基因密切聚类，与同时在武汉检测到的 P[8]和 G2P[4]株的基因距离较远。相比之下，VP1、VP6 和 NSP4 基因与典型的 G2P[4]轮状病毒密切相关，包括同时在武汉检测到的 G2P[4]株的基因。在 COVID-19 大流行时代，RVA 的检出率下降。推断在中国出现的 G8P[8]轮状病毒可能是重组体，携带 VP6、VP1 和 NSP4 基因，源自以 CAU17L-103 为代表的邻近 DS-1 样 G8P[8]株的 G2P[4]轮状病毒的骨干。
